Impaired keratinocyte function on matrix metalloproteinase-1 (MMP-1) damaged collagen
- 8 April 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für dermatologische Forschung
- Vol. 301 (7), 497-506
- https://doi.org/10.1007/s00403-009-0948-4
Abstract
Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.Keywords
This publication has 30 references indexed in Scilit:
- Vascular tube formation on matrix metalloproteinase-1-damaged collagenBritish Journal of Cancer, 2008
- Regulation of E‐cadherin and β‐catenin by Ca2+ in colon carcinoma is dependent on calcium‐sensing receptor expression and functionInternational Journal of Cancer, 2007
- Decreased Collagen Production in Chronologically Aged Skin: Roles of Age-Dependent Alteration in Fibroblast Function and Defective Mechanical StimulationThe American Journal of Pathology, 2006
- Use of an in vitro model of tissue-engineered human skin to study keratinocyte attachment and migration in the process of reepithelializationWound Repair and Regeneration, 2006
- Differential Activation of Migration by Hypoxia in Keratinocytes Isolated from Donors of Increasing Age: Implication for Chronic Wounds in the ElderlyJournal of Investigative Dermatology, 2001
- Annexin V used for measuring apoptosis in the early events of cellular cytotoxicityCytometry, 1999
- Cutaneous Wound HealingThe New England Journal of Medicine, 1999
- Intrinsically Aged Epidermis Displays Diminished UVB-Induced Alterations in Barrier Function Associated with Decreased ProliferationJournal of Investigative Dermatology, 1997
- Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.The Journal of cell biology, 1988
- Growth factor responsiveness declines during adulthood for human skin-derived cellsMechanisms of Ageing and Development, 1986