Identification and mapping of central pair proteins by proteomic analysis
Open Access
- 19 August 2019
- preprint content
- Published by Cold Spring Harbor Laboratory
- p. 739383
- https://doi.org/10.1101/739383
Abstract
Cilia or flagella of eukaryotes are small micro-hair like structures that are indispensable to single-cell motility and play an important role in mammalian biological processes. Cilia or flagella are composed of nine doublet microtubules surrounding a pair of singlet microtubules called the central pair (CP). Together, this arrangement forms the canonical and highly conserved 9+2 axonemal structure. The CP, which is a unique structure exclusive to motile cilia, is a pair of structurally dimorphic singlet microtubules decorated with numerous associated proteins. Mutations of CP-associated proteins cause several different physical symptoms termed ciliopathies. Thus, it is crucial to understand the architecture of the CP. However, the protein composition of the CP was poorly understood. This was because identification of CP proteins was mostly limited by available Chlamydomonas mutants of CP proteins. In this study, we conducted a comprehensive CP proteome analysis using several CP mutants and identified 37 novel CP protein candidates. By using Chlamydomonas strains lacking specific CP sub-structures, we also present a more complete model of localization of known and newly identified CP proteins. This work has established a new foundation for CP protein analysis for future studies.Keywords
This publication has 42 references indexed in Scilit:
- Conserved structural motifs in the central pair complex of eukaryotic flagellaCytoskeleton, 2012
- Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub‐complex members in regulating flagellar beat frequencyCytoskeleton, 2012
- Ciliopathies: an expanding disease spectrumPediatric Nephrology, 2011
- Pcdp1 is a central apparatus protein that binds Ca2+-calmodulin and regulates ciliary motilityThe Journal of cell biology, 2010
- Mutations inHydinimpair ciliary motility in miceThe Journal of cell biology, 2008
- Making sense of cilia and flagellaThe Journal of cell biology, 2007
- Chlamydomonas reinhardtiihydin is a central pair protein required for flagellar motilityThe Journal of cell biology, 2007
- ATP Production inChlamydomonas reinhardtiiFlagella by Glycolytic EnzymesMolecular Biology of the Cell, 2005
- Proteomic analysis of a eukaryotic ciliumThe Journal of cell biology, 2005
- A Sperm-Associated WD Repeat Protein Orthologous to Chlamydomonas PF20 Associates with Spag6, the Mammalian Orthologue of Chlamydomonas PF16Molecular and Cellular Biology, 2002