Chondrocyte Matrix Metalloproteinase-8: HUMAN ARTICULAR CHONDROCYTES EXPRESS NEUTROPHIL COLLAGENASE
Open Access
- 3 May 1996
- journal article
- Published by Elsevier BV
- Vol. 271 (18), 11023-11026
- https://doi.org/10.1074/jbc.271.18.11023
Abstract
This study confirms that normal human articular chondrocytes express neutrophil collagenase or matrix metalloproteinase-8 (MMP-8), a gene product previously thought to be expressed exclusively by neutrophil leukocytes. Both MMP-8 protein and mRNA were present in articular cartilages collected from normal human donors. Cartilage extracts were assayed by immunoblotting and by analysis of enzymatic activity on gelatin-substrate gels. Latent MMP-8 extracted from cartilage has a molecular mass of 55 kDa; active MMP-8 was identified at 46 and 42 kDa. In the absence of a reducing agent, MMP-8 migrated in a high molecular mass complex above 200 kDa. Northern blotting results demonstrated the expression of MMP-8 in chondrocytes, which could be up-regulated by stimulation with interleukin-1β. In addition, reverse transcription-polymerase chain reaction using nested primers and in situ hybridization revealed the presence of MMP-8 mRNA in chondrocytes. The presence of both MMP-8 protein and message in cartilage supports the concept that neutrophil collagenase could be the enzyme described as “aggrecanase.”Keywords
This publication has 21 references indexed in Scilit:
- Cell-mediated Catabolism of AggrecanPublished by Elsevier BV ,1995
- MMP-8 (neutrophil collagenase) mRNA and aggrecanase cleavage products are present in normal and osteoarthritic human articular cartilageActa Orthopaedica, 1995
- Fibroblast and neutrophil collagenases cleave at two sites in the cartilage aggrecan interglobular domainBiochemical Journal, 1993
- The structure of aggrecan fragments in human synovial fluid. evidence that aggrecanase mediates cartilage degradation in inflammatory joint disease, joint injury, and osteoarthritisArthritis & Rheumatism, 1993
- The structure of aggrecan fragments in human synovial fluid. Evidence for the involvement in osteoarthritis of a novel proteinase which cleaves the Glu 373-Ala 374 bond of the interglobular domain.JCI Insight, 1992
- Inflammatory Phagocytes and Connective Tissue Degrading MetalloproteinasesThe American Journal of the Medical Sciences, 1991
- The immunologic relationship of human neutrophil and skin collagenasesArthritis & Rheumatism, 1987
- Granulocyte collagenase: selective digestion of type I relative to type III collagen.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Antibody to Rheumatoid Synovial CollagenaseEuropean Journal of Biochemistry, 1976
- The Glomerular Permeability Determined by Dextran Clearance Using Sephadex Gel FiltrationScandinavian Journal of Clinical and Laboratory Investigation, 1968