Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana
Open Access
- 31 December 2020
- Vol. 10 (1), 73
- https://doi.org/10.3390/plants10010073
Abstract
In Arabidopsis thaliana (Arabidopsis), the microRNA399 (miR399)/PHOSPHATE2 (PHO2) expression module is central to the response of Arabidopsis to phosphate (PO4) stress. In addition, miR399 has been demonstrated to also alter in abundance in response to salt stress. We therefore used a molecular modification approach to alter miR399 abundance to investigate the requirement of altered miR399 abundance in Arabidopsis in response to salt stress. The generated transformant lines, MIM399 and MIR399 plants, with reduced and elevated miR399 abundance respectively, displayed differences in their phenotypic and physiological response to those of wild-type Arabidopsis (Col-0) plants following exposure to a 7-day period of salt stress. However, at the molecular level, elevated miR399 abundance, and therefore, altered PHO2 target gene expression in salt-stressed Col-0, MIM399 and MIR399 plants, resulted in significant changes to the expression level of the two PO4 transporter genes, PHOSPHATE TRANSPORTER1;4 (PHT1;4) and PHT1;9. Elevated PHT1;4 and PHT1;9 PO4 transporter levels in salt stressed Arabidopsis would enhance PO4 translocation from the root to the shoot tissue which would supply additional levels of this precious cellular resource that could be utilized by the aerial tissues of salt stressed Arabidopsis to either maintain essential biological processes or to mount an adaptive response to salt stress.Keywords
This publication has 57 references indexed in Scilit:
- DRB2 Is Required for MicroRNA Biogenesis in Arabidopsis thalianaPLOS ONE, 2012
- Target mimics: an embedded layer of microRNA-involved gene regulatory networks in plantsBMC Genomics, 2012
- A Collection of Target Mimics for Comprehensive Analysis of MicroRNA Function in Arabidopsis thalianaPLoS Genetics, 2010
- The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexesRNA, 2009
- Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus‐deficiency signalling in common bean rootsPlant, Cell & Environment, 2008
- MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasisThe Plant Journal, 2007
- Target mimicry provides a new mechanism for regulation of microRNA activityNature Genetics, 2007
- Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAsPlant Methods, 2007
- PHO2, MicroRNA399, and PHR1 Define a Phosphate-Signaling Pathway in PlantsPlant Physiology, 2006
- pho2, a Phosphate Overaccumulator, Is Caused by a Nonsense Mutation in a MicroRNA399 Target GenePlant Physiology, 2006