Prevention of lipopolysaccharide-induced intussusception in mice by the COX2 inhibitor rofecoxib
- 6 November 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Pediatric Surgery International
- Vol. 24 (3), 333-336
- https://doi.org/10.1007/s00383-007-2066-8
Abstract
Intussusception (IS), an invagination of a portion of the intestine into itself, has recently attracted considerable interest after the withdrawal of a rotavirus vaccine because of reports on increased risk of IS shortly after vaccination. The present study was designed to shed further light on the mechanism of IS formation and its prevention. Intussusception was induced in adult mice by intraperitoneal injection of lipopolysaccharide (LPS; 8 mg/kg) from salmonella typhimurium. The presence of IS was confirmed at laparotomy. The serum levels TNF-α were measured with ELISA. Six hours after LPS injection, 14.5% of the animals demonstrated IS. A total of 65 animals received rofecoxib (20 mg/kg), a selective COX2 inhibitor, 15–30 min before intraperitoneal injection of LPS, and only two (3%) in this group demonstrated IS 6 h later (P < 0.05 vs. control). We confirmed the well-known increase in serum TNF-α levels in response to LPS; however, this increase was not blocked by rofecoxib pretreatment. Notably, there was no correlation between the serum TNF-α levels and the development of IS. The results show that the occurrence of IS can be significantly decreased by pretreatment with a selective COX-2 inhibitor.Keywords
This publication has 21 references indexed in Scilit:
- A critical time for rotavirus vaccines: a reviewExpert Review of Vaccines, 2005
- Intussusception in Early Childhood: A Cohort Study of 1.7 Million ChildrenPEDIATRICS, 2004
- Endotoxin pretreatment modifies peristalsis and attenuates the antipropulsive action of adrenoceptor agonists in the guinea-pig small intestineNeurogastroenterology & Motility, 2004
- IntussusceptionPediatric Radiology, 2004
- Endotoxin, cytokines and lipid peroxides in children with intussusceptionBritish Journal of Surgery, 2001
- Involvement of Nitric Oxide and Prostaglandins in Gastroprotection Induced by Bacterial LipopolysaccharideScandinavian Journal of Gastroenterology, 1998
- The Contribution of Inflammatory Mediators and Nitric Oxide to Lipopolysaccharide-Induced Intussusception in MiceJournal of Surgical Research, 1997
- Expression of inducible cyclooxygenase mRNA in the mouse brain after systemic administration of bacterial lipopolysaccharideBrain Research, 1996
- Interleukin-1-Induced Prostaglandin E2 Biosynthesis in Human Synovial Cells Involves the Activation of Cytosolic Phospholipase A2 and Cyclooxygenase-2JBIC Journal of Biological Inorganic Chemistry, 1994
- Paediatric intussusceptionBritish Journal of Surgery, 1992