A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552
Open Access
- 3 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 13 (1), 1-12
- https://doi.org/10.1186/1471-2180-13-96
Abstract
Many Gram-negative bacteria rely on a type VI secretion system (T6SS) to infect eukaryotic cells or to compete against other microbes. Common to these systems is the presence of two conserved proteins, in Vibrio cholerae denoted VipA and VipB, which have been shown to interact in many clinically relevant pathogens. In this study, mutagenesis of a defined region within the VipA protein was used to identify residues important for VipB binding in V. cholerae O1 strain A1552. A dramatically diminished interaction was shown to correlate with a decrease in VipB stability and a loss of hemolysin co-regulated protein (Hcp) secretion and rendered the bacterium unable to compete with Escherichia coli in a competition assay. This confirms the biological relevance of the VipA-VipB interaction, which is essential for the T6SS activity of many important human pathogens.Keywords
This publication has 63 references indexed in Scilit:
- Disruption of a rice gene for α-glucan water dikinase, OsGWD1, leads to hyperaccumulation of starch in leaves but exhibits limited effects on growthFrontiers in Plant Science, 2013
- Type VI secretion delivers bacteriolytic effectors to target cellsNature, 2011
- What is type VI secretion doing in all those bugs?Trends in Microbiology, 2010
- Type VI Secretion: Not Just for Pathogenesis AnymoreCell Host & Microbe, 2010
- A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to BacteriaCell Host & Microbe, 2010
- Translocation of a Vibrio cholerae Type VI Secretion Effector Requires Bacterial Endocytosis by Host CellsCell Host & Microbe, 2009
- Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for type VI protein secretionThe EMBO Journal, 2009
- The type VI secretion toolkitEMBO Reports, 2008
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- A simple and efficient method for isolating RNA from pine treesPlant Molecular Biology Reporter, 1993