Ribosomal protein L7Ae is a subunit of archaeal RNase P
- 30 July 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (33), 14573-14578
- https://doi.org/10.1073/pnas.1005556107
Abstract
To the mounting evidence of nonribosomal functions for ribosomal proteins, we now add L7Ae as a subunit of archaeal RNase P, a ribonucleoprotein (RNP) that catalyzes 5′-maturation of precursor tRNAs (pre-tRNAs). We first demonstrate that L7Ae coelutes with partially purified Methanococcus maripaludis (Mma) RNase P activity. After establishing in vitro reconstitution of the single RNA with four previously known protein subunits (POP5, RPP21, RPP29, and RPP30), we show that addition of L7Ae to this RNase P complex increases the optimal reaction temperature and kcat/Km (by ∼360-fold) for pre-tRNA cleavage to those observed with partially purified native Mma RNase P. We identify in the Mma RNase P RNA a putative kink-turn (K-turn), the structural motif recognized by L7Ae. The large stimulatory effect of Mma L7Ae on RNase P activity decreases to ≤ 4% of wild type upon mutating either the conserved nucleotides in this K-turn or amino acids in L7Ae shown to be essential for K-turn binding. The critical, multifunctional role of archaeal L7Ae in RNPs acting in tRNA processing (RNase P), RNA modification (H/ACA, C/D snoRNPs), and translation (ribosomes), especially by employing the same RNA-recognition surface, suggests coevolution of various translation-related functions, presumably to facilitate their coordinate regulation.This publication has 46 references indexed in Scilit:
- Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complexNucleic Acids Research, 2010
- Unexpected diversity of RNase P, an ancient tRNA processing enzyme: Challenges and prospectsFEBS Letters, 2009
- Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysisNucleic Acids Research, 2008
- The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machineryThe Journal of cell biology, 2008
- Eukaryotic RNase P RNA mediates cleavage in the absence of proteinProceedings of the National Academy of Sciences of the United States of America, 2007
- Functional reconstitution and characterization of Pyrococcus furiosus RNase PProceedings of the National Academy of Sciences of the United States of America, 2006
- Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymesNucleic Acids Research, 2006
- Evidence that substrate-specific effects of C5 protein lead to uniformity in binding and catalysis by RNase PThe EMBO Journal, 2006
- Ribonuclease P: The Evolution of an Ancient RNA EnzymeCritical Reviews in Biochemistry and Molecular Biology, 2006
- The kink-turn: a new RNA secondary structure motifThe EMBO Journal, 2001