Broad Spectrum Respiratory Pathogen Analysis of Throat Swabs from Military Recruits Reveals Interference Between Rhinoviruses and Adenoviruses
- 9 March 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Microbial Ecology
- Vol. 59 (4), 623-634
- https://doi.org/10.1007/s00248-010-9636-3
Abstract
Military recruits experience a high incidence of febrile respiratory illness (FRI), leading to significant morbidity and lost training time. Adenoviruses, group A Streptococcus pyogenes, and influenza virus are implicated in over half of the FRI cases reported at recruit training center clinics, while the etiology of the remaining cases is unclear. In this study, we explore the carriage rates and disease associations of adenovirus, enterovirus, rhinovirus, Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis in military recruits using high-density resequencing microarrays. The results showed that rhinoviruses, adenoviruses, S. pneumoniae, H. influenzae, and N. meningitidis were widely distributed in recruits. Of these five agents, only adenovirus showed significant correlation with illness. Among the samples tested, only pathogens associated with FRI, such as adenovirus 4 and enterovirus 68, revealed strong temporal and spatial clustering of specific strains, indicating that they are transmitted primarily within sites. The results showed a strong negative association between adenoviral FRI and the presence of rhinoviruses in recruits, suggesting some form of viral interference.Keywords
This publication has 47 references indexed in Scilit:
- Universal Detection and Identification of Avian Influenza Virus by Use of Resequencing MicroarraysJournal of Clinical Microbiology, 2009
- Microbial Interactions during Upper Respiratory Tract InfectionsEmerging Infectious Diseases, 2008
- Resequencing microarray probe design for typing genetically diverse viruses: human rhinoviruses and enterovirusesBMC Genomics, 2008
- Comparison of Results of Detection of Rhinovirus by PCR and Viral Culture in Human Nasal Wash Specimens from Subjects with and without Clinical Symptoms of Respiratory IllnessJournal of Clinical Microbiology, 2007
- Application of Broad-Spectrum, Sequence-Based Pathogen Identification in an Urban PopulationPLOS ONE, 2007
- Meningococcal Genetic Variation Mechanisms Viewed through Comparative Analysis of Serogroup C Strain FAM18PLoS Genetics, 2007
- Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection AssayJournal of Clinical Microbiology, 2007
- Automated identification of multiple micro-organisms from resequencing DNA microarraysNucleic Acids Research, 2006
- Identifying Influenza Viruses with Resequencing MicroarraysEmerging Infectious Diseases, 2006
- Streptococcus pneumoniae colonisation: the key to pneumococcal diseaseThe Lancet Infectious Diseases, 2004