Distinct Expression Profiles and Different Functions of Odorant Binding Proteins in Nilaparvata lugens Stål
Open Access
- 9 December 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (12), e28921
- https://doi.org/10.1371/journal.pone.0028921
Abstract
Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in BPH behavior. However, the olfaction related proteins have not been characterized in this insect. Full length cDNA of three OBPs were obtained and distinct expression profiles were revealed regarding to tissue, developmental stage, wing form and gender for the first time for the species. The results provide important clues in functional differentiation of these genes. Binding assays with 41 compounds demonstrated that NlugOBP3 had markedly higher binding ability and wider binding spectrum than the other two OBPs. Terpenes and Ketones displayed higher binding while Alkanes showed no binding to the three OBPs. Focused on NlugOBP3, RNA interference experiments showed that NlugOBP3 not only involved in nymph olfaction on rice seedlings, but also had non-olfactory functions, as it was closely related to nymph survival. NlugOBP3 plays important roles in both olfaction and survival of BPH. It may serve as a potential target for developing behavioral disruptant and/or lethal agent in N. lugens.Keywords
This publication has 48 references indexed in Scilit:
- Functional dissection of Odorant binding protein genes in Drosophila melanogasterGenes, Brain and Behavior, 2011
- Feeding‐based RNA interference of a trehalose phosphate synthase gene in the brown planthopper, Nilaparvata lugensInsect Molecular Biology, 2010
- Knockdown of a Mosquito Odorant-binding Protein Involved in the Sensitive Detection of Oviposition AttractantsJournal of Chemical Ecology, 2010
- Large-scale identification of odorant-binding proteins and chemosensory proteins from expressed sequence tags in insectsBMC Genomics, 2009
- Jalview Version 2—a multiple sequence alignment editor and analysis workbenchBioinformatics, 2009
- Molecular cloning and characterization of a juvenile hormone esterase gene from brown planthopper, Nilaparvata lugensJournal of Insect Physiology, 2008
- Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens: a genomic resource for studying agricultural pestsBMC Genomics, 2008
- Clustal W and Clustal X version 2.0Bioinformatics, 2007
- Locating proteins in the cell using TargetP, SignalP and related toolsNature Protocols, 2007
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001