Alkaloid production by hairy root cultures in Atropa belladonna
- 1 August 1986
- journal article
- research article
- Published by Springer Science and Business Media LLC in Plant Cell Reports
- Vol. 5 (4), 239-242
- https://doi.org/10.1007/bf00269811
Abstract
Hairy roots were induced by inoculation of stems of sterile plants of Atropa belladonna with Agrobacterium rhizogenes. The axenic culture of the hairy roots isolated from the stems proliferated 60 fold as based on the initial fresh weight after one month of culture. The presence of atropine and scopolamine in hairy roots were examined by TLC and HPLC. Their amounts were analyzed by GLC. The results show that the amount of the two alkaloids in the axenic cultures was the same as or even higher than those of normal plants grown in the field.Keywords
This publication has 19 references indexed in Scilit:
- Instability of Indole Alkaloid Production inCatharanthus roseusCell Suspension CulturesPlanta Medica, 1984
- Transformation of several species of higher plants by agrobacterium rhizogenes: Sexual transmission of the transformed genotype and phenotypeCell, 1984
- Scopolamine Production in Suspension Cultures and Redifferentiated Roots ofHyoscyamus nigerPlanta Medica, 1983
- Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cellsNature, 1982
- A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activitiesBiochimica et Biophysica Acta (BBA) - Enzymology, 1977
- Characterization of Different Plaque-forming and Defective Temperate Phages in Agrobacterium StrainsJournal of General Virology, 1975
- Alkaloid production by plants regenerated from cultured cells of Datura innoxiaPhytochemistry, 1974
- Organization and alkaloid production in tissue cultures of scopolia parvifloraPhytochemistry, 1972
- Synthesis of Atropine by Isolated Roots and Root-Callus Cultures of BelladonnaBotanical Gazette, 1957
- Detection of Sugars on Paper ChromatogramsNature, 1950