Directed Biosynthesis of Alkaloid Analogs in the Medicinal Plant Catharanthus roseus
- 18 October 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (44), 14276-14277
- https://doi.org/10.1021/ja066787w
Abstract
Terpene indole alkaloids are plant natural products with diverse structures and biological activities. A highly branched biosynthetic pathway is responsible for the production of approximately 130 different alkaloids in Madagascar periwinkle (C. roseus) from a common biosynthetic intermediate derived from tryptamine. Although numerous biosynthetic pathways can incorporate unnatural starting materials to yield novel natural products, it was not clear how efficiently the complex, eukaryotic TIA pathway could utilize unnatural substrates to make new alkaloids. This work demonstrates that the TIA biosynthetic machinery can be used to produce novel alkaloid structures and also highlights the potential of this pathway for future metabolic engineering efforts.Keywords
This publication has 8 references indexed in Scilit:
- Substrate specificity of strictosidine synthaseBioorganic & Medicinal Chemistry Letters, 2006
- Alkaloid accumulation in Catharanthus roseus cell suspension cultures fed with stemmadenineBiotechnology Letters, 2004
- Vindoline Biosynthesis Is Transcriptionally locked in Catharanthus roseus Cell Suspension CulturesMolecular Biotechnology, 2002
- Metabolic engineering and directed evolution for the production of pharmaceuticalsCurrent Opinion in Biotechnology, 2000
- Variation in vinblastine production by Catharanthus roseus, during in vivo and in vitro differentiationPhytochemistry, 1997
- Altering Flavor Profiles in Onion (Allium cepa L.) Root Cultures Through Directed BiosynthesisBiotechnology Progress, 1997
- Production of indole alkaloids by selected hairy root lines of Catharanthus roseusBiotechnology & Bioengineering, 1993
- Microtubule Crystals: A New Biophysical Phenomenon induced by Vinca AlkaloidsNature, 1968