Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
Open Access
- 10 March 2020
- preprint content
- other
- Published by Cold Spring Harbor Laboratory
Abstract
An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China in December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report the development of SARS-CoV-2 DETECTR, a rapid (∼30 min), low-cost, and accurate CRISPR-Cas12 based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated this method using contrived reference samples and clinical samples from infected US patients and demonstrated comparable performance to the US CDC SARS-CoV-2 real-time RT-PCR assay.Keywords
This publication has 15 references indexed in Scilit:
- Community Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, Shenzhen, China, 2020Emerging Infectious Diseases, 2020
- Presumed Asymptomatic Carrier Transmission of COVID-19Jama-Journal Of The American Medical Association, 2020
- Transmission of 2019-nCoV Infection from an Asymptomatic Contact in GermanyThe New England Journal of Medicine, 2020
- A Novel Coronavirus from Patients with Pneumonia in China, 2019The New England Journal of Medicine, 2020
- A novel coronavirus outbreak of global health concernThe Lancet, 2020
- Cutting-Edge Infectious Disease Diagnostics with CRISPRCell Host & Microbe, 2018
- Field-deployable viral diagnostics using CRISPR-Cas13Science, 2018
- CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activityScience, 2018
- Nucleic acid detection with CRISPR-Cas13a/C2c2Science, 2017
- Loop-mediated isothermal amplification of DNANucleic Acids Research, 2000