Human Papillomavirus DNA in Plasma of Patients with HPV16 DNA-positive Uterine Cervical Cancer
- 4 February 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Japanese Journal of Clinical Oncology
- Vol. 40 (5), 420-424
- https://doi.org/10.1093/jjco/hyp193
Abstract
The squamous cell carcinoma antigen is considered the most accurate serologic tumor marker for uterine cervical carcinoma. However, serum squamous cell carcinoma antigen levels were found to correlate significantly with clinical severity of atopic dermatitis and chronic renal failure. The present study was conducted in patients with human papillomavirus 16 DNA-positive uterine cervical cancer to determine the plasma level of human papillomavirus 16 DNA and the diagnostic values of plasma human papillomavirus DNA in these patients.Forty-three human papillomavirus 16-positive patients with cervical intraepithelial neoplasia or uterine cervical squamous cell carcinoma were recruited in this study. The diagnosis was cervical cancer in 20 patients, high-grade squamous intraepithelial lesions in 21, low-grade squamous intraepithelial lesions in 1 and negative for intraepithelial lesion or malignancy in 3 patients. Before any treatment, blood samples were collected from all patients. For analysis of human papillomavirus DNA in plasma of patients with cervical cancer, quantitative polymerase chain reaction fluorescent assay for human papillomavirus 16 was performed using human papillomavirus 16 primers and SYBR Green dye using the LightCycler 480 SW1.5 apparatus.Plasma human papillomavirus 16 DNA was detected in only 30.0% of the patients with human papillomavirus 16-positive cervical cancer and in none of normal controls. The copy number of plasma human papillomavirus 16 DNA was higher in patients with invasive cancer than in those with cervical intraepithelial neoplasia (CIN3), micro-invasive cancer and in normal individuals.These results indicated that the plasma human papillomavirus DNA level could be potentially used as a marker of low-invasive cervical cancer tumors in patients with normal squamous cell carcinoma antigen levels before treatment.Keywords
This publication has 14 references indexed in Scilit:
- Detection and quantitation of human papillomavirus type 16, 18 and 52 DNA in the peripheral blood of cervical cancer patientsGynecologic Oncology, 2005
- The squamous cell carcinoma antigens as relevant biomarkers of atopic dermatitisClinical and Experimental Allergy, 2005
- Quantification of human papillomavirus DNA in the plasma of patients with cervical cancerInternational Journal of Gynecologic Cancer, 2004
- Type specific persistence of high risk human papillomavirus (HPV) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up studyBMJ, 2002
- Human papillomavirus DNA in plasma of patients with cervical cancerBMC Cancer, 2001
- The Prevalence of Genital Human Papillomavirus Infections in Abused and Nonabused Preadolescent GirlsPEDIATRICS, 2000
- Epidemiology of Acquisition and Clearance of Cervical Human Papillomavirus Infection in Women from a High‐Risk Area for Cervical CancerThe Journal of Infectious Diseases, 1999
- Perinatal infection and persistence of human papillomavirus types 16 and 18 in infantsJournal of Medical Virology, 1995
- Evaluation of Sexually Abused and Nonabused Young Girls for Intravaginal Human Papillomavirus InfectionArchives of Pediatrics & Adolescent Medicine, 1992
- Radioimmunoassay for tumor antigen of human cervical squamous cell carcinomaCancer, 1977