Hepatitis C virus NS5A protein protects against TNF-α mediated apoptotic cell death
- 1 April 2000
- journal article
- Published by Elsevier BV in Virus Research
- Vol. 67 (2), 173-178
- https://doi.org/10.1016/s0168-1702(00)00141-6
Abstract
Hepatitis C virus (HCV) often causes a prolonged and persistent infection which may lead to hepatocellular carcinoma. We have previously reported that the nonstructural 5A (NS5A) protein of HCV promotes cell growth [Ghosh, A.K., Steele, R., Meyer, K., Ray, R., Ray, R.B., 1999. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth. J. Gen. Virol. 80, 1179–1183]. In this study, we investigated the role of HCV NS5A (genotype 1a, strain H) in TNF-α induced apoptotic cell death. HepG2 cells expressing NS5A exhibited an inhibitory role in relation to TNF-α mediated apoptotic cell death. The NS5A protein blocked the activation of caspase-3 and inhibited proteolytic cleavage of the death substrate poly (ADP-ribose) polymerase in TNF-α induced cells. Together, these results suggest that HCV NS5A protein protects against TNF-α mediated apoptotic cell death.Keywords
This publication has 22 references indexed in Scilit:
- Hepatitis C Virus Core Protein Inhibits Fas- and Tumor Necrosis Factor Alpha-Mediated Apoptosis via NF-κB ActivationJournal of Virology, 1999
- Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth.Journal of General Virology, 1999
- Phosphorylation of Nonstructural 5A Protein of Hepatitis C Virus: HCV Group-Specific HyperphosphorylationVirology, 1999
- Characterization of Transgenic Mice with Targeted Disruption of the Catalytic Domain of the Double-stranded RNA-dependent Protein Kinase, PKRPublished by Elsevier BV ,1999
- Hepatitis C virus NS5A protein is phosphorylated in vitro by a stably bound protein kinase from HeLa cells and by cAMP-dependent protein kinase A-α catalytic subunitGene, 1997
- Apoptosis by Death FactorCell, 1997
- An Essential Role for NF-κB in Preventing TNF-α-Induced Cell DeathScience, 1996
- Viral oncogenesis and cell cycle controlVirus Research, 1996
- Comparison of full-length sequences of interferon-sensitive and resistant hepatitis C virus 1b. Sensitivity to interferon is conferred by amino acid substitutions in the NS5A region.JCI Insight, 1995
- Production of Two Phosphoproteins from the NS5A Region of the Hepatitis C Viral GenomeBiochemical and Biophysical Research Communications, 1994