FUNCTIONS OF COATED VESICLES DURING PROTEIN ABSORPTION IN THE RAT VAS DEFERENS
Open Access
- 1 November 1967
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 35 (2), 357-376
- https://doi.org/10.1083/jcb.35.2.357
Abstract
The role of coated vesicles during the absorption of horseradish peroxidase was investigated in the epithelium of the rat vas deferens by electron microscopy and cytochemistry. Peroxidase was introduced into the vas lumen in vivo. Tissue was excised at selected intervals, fixed in formaldehyde-glutaraldehyde, sectioned without freezing, incubated in Karnovsky's medium, postfixed in OsO4, and processed for electron microscopy. Some controls and peroxidase-perfused specimens were incubated with TPP,1 GP, and CMP. Attention was focused on the Golgi complex, apical multivesicular bodies, and two populations of coated vesicles; large (> 1000 A) ones concentrated in the apical cytoplasm and small (a) this epithelium functions in the absorption of protein from the duct lumen, (b) large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and (c) some small coated vesicles serve as primary lysosomes to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.Keywords
This publication has 28 references indexed in Scilit:
- Absorption of ferritin by rat kidney proximal tubule cells: Electron microscopic observations of the initial uptake phase in cells of microperfused single proximal tubulesJournal of Ultrastructure Research, 1966
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966
- THE ENTERIC SURFACE COAT ON CAT INTESTINAL MICROVILLIThe Journal of cell biology, 1965
- TRANSPORT AND DIGESTION OF HEMOGLOBIN IN THE PROXIMAL TUBULE. II. ELECTRON MICROSCOPY.1965
- THE DISTRIBUTION OF EXOGENOUS FERRITIN IN TOAD SPINAL GANGLIA AND THE MECHANISM OF ITS UPTAKE BY NEURONSThe Journal of cell biology, 1964
- Uptake of colloidal particles by cells of the caput epididymidisThe Anatomical Record, 1964
- JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIAThe Journal of cell biology, 1963
- The granule cells, mossy synapses and Purkinje spine synapses of the cerebellum: light and electron microscope observations.1961
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961
- Segregation of Ferritin in Glomerular Protein Absorption DropletsThe Journal of cell biology, 1960