The incidence and size of gap junctions between the bone cells in rat calvaria
- 1 April 1993
- journal article
- Published by Springer Science and Business Media LLC in Brain Structure and Function
- Vol. 187 (4), 343-352
- https://doi.org/10.1007/bf00185892
Abstract
Polyclonal antisera to synthetic peptides matching sequences on the cytoplasmic regions of connexin-43, a gap junction protein first identified in rat heart, have been used to immunolabel gap junctions in the calvarial bone, maintained intact as in vivo, of 1- to 2-week-old rats. The specimens were examined in reflection and fluorescence modes by scanning laser confocal microscopy, and the numbers of gap junctions and their sizes estimated. The mean number of connexin-43 immunolabelled junctions per osteoblast (n=65) was 15.3 (SD ± 4.5). The mean length of 227 junctions, selected for the sharpness of the image of the fluorescent spot, was 0.67 μm (SD ± 0.18; range 0.37–1.29 μm) and their mean area 0.26 μm2 (SD ± 0.145; range 0.075–0.93 μm2); these probably fell within the upper half of the total size range. Gap junctions were detected between preosteoblasts, osteoblasts, osteocytes and chondrocytes, and between these juxtaposed cell types. In addition, connexin-43 immuno-labelled junctions were found between some osteoclasts and overlying mononuclear cells at active sites of resorption.Keywords
This publication has 23 references indexed in Scilit:
- Gap junctional communication during limb cartilage differentiationDevelopmental Biology, 1991
- Membrane modifications in chick osteoclasts revealed by freeze‐fracture replicasJournal of Anatomy, 1990
- Confocal fluorescence microscopy: some applications in bone cell biologyJournal of Microscopy, 1990
- Scrape-loading and dye transferExperimental Cell Research, 1987
- Prostaglandins change cell shape and increase intercellular gap junctions in osteoblasts cultured from rat fetal calvariaJournal of Bone and Mineral Research, 1986
- Constants in lamellar bone formation determined by osteoblast kineticsBone, 1985
- Scanning electron microscopic observations of rat tibia using the HCl-collagenase method.Archivum histologicum japonicum, 1982
- Secretory territories and rate of matrix production of osteoblastsCalcified Tissue International, 1974
- An ultrastructural study of bone cells: The occurrence of microtubules, microfilaments and tight junctionsCalcified Tissue International, 1974
- Tight junctions between osteocyte processesEuropean Journal of Oral Sciences, 1973