Zea mI, the maize homolog of the allergen-encoding Lol pI gene of rye grass
- 1 September 1993
- journal article
- research article
- Published by Elsevier BV in Gene
- Vol. 131 (2), 227-230
- https://doi.org/10.1016/0378-1119(93)90297-g
Abstract
Sequence analysis of a pollen-specific cDNA from maize has identified a homolog (Zea mI) of the gene (Lol pI) encoding the major allergen of rye-grass pollen. The protein encoded by the partial cDNA sequence is 59.3% identical and 72.7% similar to the comparable region of the reported amino acid sequence of Lol pIA. Southern analysis indicates that this cDNA represents a member of a small multigene family in maize. Northern analysis shows expression only in pollen, not in vegetative or female floral tissues. The timing of expression is developmentally regulated, occuring at a low level prior to the first pollen mitosis and at a high level after this postmeiotic division. Western analysis detects a protein in maize pollen lysates using polyclonal antiserum and monoclonal antibodies directed against purified Lolium perenne allergen.Keywords
This publication has 13 references indexed in Scilit:
- Olive (Olea europea) and privet (Ligustrum vulgare) pollen allergens. Identification and cross-reactivity with grass pollen proteinsMolecular Immunology, 1992
- Purification of Maize Pollen Exines and Analysis of Associated ProteinsPlant Physiology, 1992
- Cloning sequencing of Lol pI, the major allergenic protein of rye‐grass pollenFEBS Letters, 1991
- Developmental Staging of Maize Microspores Reveals a Transition in Developing Microspore ProteinsPlant Physiology, 1990
- Isolation and characterization of a major cross-reactive grass group I allergenic determinantMolecular Immunology, 1989
- Epidemiology of the Allergic Diseases: Are They Really on the Increase?International Archives of Allergy and Immunology, 1989
- Genes Expressed in the Male Gametophyte of Flowering Plants and Their IsolationPlant Physiology, 1987
- A study of the human immune response to Lolium perenne (Rye) pollen and its components, Lolp I and Lolp II (Rye I and Rye II): I. Prevalence of reactivity to the allergens and correlations among skin test, IgE antibody, and IgG antibody dataJournal of Allergy and Clinical Immunology, 1986
- A Re-examination of Ryegrass (Lolium perenne) Pollen AllergensInternational Archives of Allergy and Immunology, 1986
- Onset of Alcohol Dehydrogenase Synthesis during Microsporogenesis in MaizePlant Physiology, 1985