Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus
- 1 May 1987
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 22 (1), 45-56
- https://doi.org/10.1002/jmv.1890220107
Abstract
Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM-175 from persistently infected, serially passed cell cultures (pHM-175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM-175 in FRhK-4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.Keywords
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