Formation of Differentiated Tissues in vivo by Periodontal Cell Populations Cultured in vitro
- 1 May 1995
- journal article
- research article
- Published by SAGE Publications in Journal of Dental Research
- Vol. 74 (5), 1219-1225
- https://doi.org/10.1177/00220345950740051201
Abstract
The periodontium contains heterogeneous mesenchymal cell populations with various differentiation potentials. The capacity of these cells for tissue formation as well as the origin of their precursors are still not entirely defined. In this study, cells originating from different periodontal tissues were cultured in vitro, and tissue formation in vivo following orthotopic re-implantation was investigated. Cells were recovered from the alveolar bone and periodontal ligament tissue of six minipigs, and cultured cells were then grown on extracted dental roots from the homologous animals by means of co-culture in vitro. Each minipig received 2 roots covered with alveolar bone cells, 2 roots covered with periodontal ligament cells, and 2 control roots (without cells) implanted into palatal bone defects. Intravital fluorochrome labeling was performed, and two minipigs were histologically examined after 2, 4, and 12 weeks in each case. Controls showed widespread resorption and ankylosis, whereas roots covered with cultured periodontal cells exhibited tissue formation in vivo. Alveolar bone cells synthesized a calcified cellular tissue resembling cellular cementum, suggesting that cells within this population might differentiate into cementoblasts when re-implanted with a dental substrate in vivo. Periodontal ligament cells exhibited no calcified tissue formation in vivo, but cells synthesized a connective tissue with orientated fiber bundles attached to both host bone and root, resembling periodontal ligament. Results indicate that cultured cells retain the capacity to form differentiated periodontal tissues after re-implantation, depending on the cells' origin. Our findings also suggest that the alveolar bone contains cementogenic precursors with the potential to differentiate into active cementoblasts in the presence of a dental substrate.Keywords
This publication has 28 references indexed in Scilit:
- The collagens and glycosaminoglycans of the extracellular matrices secreted by bone marrow stromal cells cultured in vivo in diffusion chambersJournal of Orthopaedic Research, 1990
- Re-implantation of homologous, cultivated osteoblast-like cells for improvement of bone regeneration: An animal studyInternational Journal of Oral & Maxillofacial Surgery, 1989
- Long-term culture of human periodontal ligament cells with autologous root discsJournal of Periodontal Research, 1988
- Synthesis of cementum‐like tissue in vitro by cells cultured from bone: a light and electron microscope studyJournal of Periodontal Research, 1986
- The influence of the morphological and chemical nature of dental surfaces on the migration, attachment, and orientation of human gingival fibroblasts in vitroJournal of Periodontal Research, 1984
- Nuclear size as a cell‐kinetic marker for osteoblast differentiationJournal of Anatomy, 1982
- A method for the study of undecalcified bones and teeth with attached soft tissues*Journal of Oral Pathology & Medicine, 1982
- Formation of new periodontalligament by periodontal ligament cells implanted in vivo after culture in vitroJournal of Periodontal Research, 1981
- The estimation of two collagens from human dermis by interrupted gel electrophoresisBiochemical and Biophysical Research Communications, 1976
- On the Repair Potential of Periodontal TissuesThe Journal of Periodontology, 1976