Alkaline Phosphatase-Catalyzed Silver Deposition for Electrochemical Detection
- 15 June 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (14), 5272-5277
- https://doi.org/10.1021/ac070624o
Abstract
Alkaline phosphatase (AP) is one of the most used enzymatic labels for the development of ELISAs, immunosensors, DNA hybridization assays, etc. This enzyme catalyzes the dephosphorylation of a substrate into a detectable product usually quantified by optical or electrochemical measurements. This work is based on a substrate (3-indoxyl phosphate) that produces a compound able to reduce silver ions in solution into a metallic deposit, which is localized where the enzymatic label AP is attached. The deposited silver is electrochemically stripped into solution and measured by anodic stripping voltammetry. Its application to an enzymatic genosensor on streptavidin-modified screen-printed carbon electrodes for the detection of virulence nucleic acid determinants of autolysin gene, exclusively present on the genome of the human pathogen Streptococcus pneumoniae, is described. Compared with the direct voltammetric detection of indigo carmine, the anodic stripping voltammetry of silver ions is 14-fold more sensitive.Keywords
This publication has 24 references indexed in Scilit:
- DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodesBiosensors and Bioelectronics, 2006
- Detection of leucoindigo in alkaline phosphatase and peroxidase based assays using 3-indoxyl phosphate as substrateAnalytica Chimica Acta, 2005
- Genosensor Based on a Platinum(II) Complex as Electrocatalytic LabelAnalytical Chemistry, 2005
- Electrochemical detection of the toxic dinoflagellate Alexandrium ostenfeldii with a DNA-biosensorBiosensors and Bioelectronics, 2005
- Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence☆Biosensors and Bioelectronics, 2004
- Enzymatic Genosensor on Streptavidin-Modified Screen-Printed Carbon ElectrodesAnalytical Chemistry, 2004
- Voltammetric determination of alkaline phosphatase and horseradish peroxidase activity using 3-indoxyl phosphate as substrate: Application to enzyme immunoassayTalanta, 2004
- Use of Denaturing HPLC for Detection of Mutations in the BCR-ABL Kinase Domain in Patients Resistant to ImatinibClinical Chemistry, 2004
- Electrochemical determination of gold nanoparticles in colloidal solutionsElectrochimica Acta, 2000
- Disposable DNA electrochemical sensor for hybridization detection1This paper was presented at the Fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1Biosensors and Bioelectronics, 1998