A transcription aptasensor: amplified, label-free and culture-independent detection of foodborne pathogens via light-up RNA aptamers
- 29 July 2019
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 55 (68), 10096-10099
- https://doi.org/10.1039/c9cc05036a
Abstract
We report a transcription aptasensor by using a light-up RNA aptamer. It allows for sensitive, label-free and culture-free detection of intact foodborne pathogens, and no separation, purification or enrichment processes are involved.Keywords
Funding Information
- National Natural Science Foundation of China (21235004, 21621003, 21804095)
- China Postdoctoral Science Foundation (2018M631079)
- National Basic Research Program of China (2016YFA0203101)
This publication has 38 references indexed in Scilit:
- Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteriaNature Protocols, 2013
- Fluorescence Imaging of Cellular Metabolites with RNAScience, 2012
- RNA Mimics of Green Fluorescent ProteinScience, 2011
- Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targetsNature Chemistry, 2011
- Foodborne Illness Acquired in the United States—Unspecified AgentsEmerging Infectious Diseases, 2011
- Foodborne Illness Acquired in the United States-Major PathogensEmerging Infectious Diseases, 2010
- In-Gel Technology for PCR Genotyping and Pathogen DetectionAnalytical Chemistry, 2010
- An overview of foodborne pathogen detection: In the perspective of biosensorsBiotechnology Advances, 2010
- Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureusNucleic Acids Research, 2009
- Environmental Monitoring for Biological Threat Agents Using the Autonomous Pathogen Detection System with Multiplexed Polymerase Chain ReactionAnalytical Chemistry, 2008