Genomic and Proteomic Analysis of Invertebrate Iridovirus Type 9
- 1 August 2011
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 85 (15), 7900-7911
- https://doi.org/10.1128/jvi.00645-11
Abstract
Iridoviruses (IV) are nuclear cytoplasmic large DNA viruses that are receiving increasing attention as sublethal pathogens of a range of insects. Invertebrate iridovirus type 9 (IIV-9; Wiseana iridovirus) is a member of the major phylogenetic group of iridoviruses for which there is very limited genomic and proteomic information. The genome is 205,791 bp, has a G+C content of 31%, and contains 191 predicted genes, with approximately 20% of its repeat sequences being located predominantly within coding regions. The repeated sequences include 11 proteins with helix-turn-helix motifs and genes encoding related tandem repeat amino acid sequences. Of the 191 proteins encoded by IIV-9, 108 are most closely related to orthologs in IIV-3 ( Chloriridovirus genus), and 114 of the 126 IIV-3 genes have orthologs in IIV-9. In contrast, only 97 of 211 IIV-6 genes have orthologs in IIV-9. There is almost no conservation of gene order between IIV-3, IIV-6, and IIV-9. Phylogenetic analysis using a concatenated sequence of 26 core IV genes confirms that IIV-3 is more closely related to IIV-9 than to IIV-6, despite being from a different genus of the Iridoviridae . An interaction between IIV and small RNA regulatory systems is supported by the prediction of seven putative microRNA (miRNA) sequences combined with XRN exonuclease, RNase III, and double-stranded RNA binding activities encoded on the genome. Proteomic analysis of IIV-9 identified 64 proteins in the virus particle and, when combined with infected cell analysis, confirmed the expression of 94 viral proteins. This study provides the first full-genome and consequent proteomic analysis of group II IIV.Keywords
This publication has 44 references indexed in Scilit:
- Identification of a Novel Marine Fish Virus, Singapore Grouper Iridovirus-Encoded MicroRNAs Expressed in Grouper Cells by Solexa SequencingPLOS ONE, 2011
- Iridovirus and Microsporidian Linked to Honey Bee Colony DeclinePLOS ONE, 2010
- Complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of IridoviridaeBMC Genomics, 2009
- The Capsid Proteins of a Large, Icosahedral dsDNA VirusJournal of Molecular Biology, 2009
- Functional characterization of the RNase III gene of rock bream iridovirusArchiv für die gesamte Virusforschung, 2008
- XSTREAM: A practical algorithm for identification and architecture modeling of tandem repeats in protein sequencesBMC Bioinformatics, 2007
- MiPred: classification of real and pseudo microRNA precursors using random forest prediction model with combined featuresNucleic Acids Research, 2007
- Genome of Invertebrate Iridescent Virus Type 3 (Mosquito Iridescent Virus)Journal of Virology, 2006
- The many faces of the helix-turn-helix domain: Transcription regulation and beyondFEMS Microbiology Reviews, 2005
- Complete Genome Sequence of Lymphocystis Disease Virus Isolated from ChinaJournal of Virology, 2004