Surface enhanced IR absorption of nucleic acids from tumor cells: FTIR reflectance study
- 1 January 2002
- journal article
- research article
- Published by Wiley in Peptide Science
- Vol. 67 (6), 470-486
- https://doi.org/10.1002/bip.10165
Abstract
The data on surface enhanced IR absorption (SEIRA) of nucleic acids deposited on a metal substrate were obtained using FTIR in reflectance mode. A 200–400 Å thick gold film on a glass plate was the metal substrate. The approximate enhancement factors of the SEIRA for different vibrations of nucleic acids in our experimental conditions were 3–5. The roughness of the Au surface was about 50 Å. Application of this method to nucleic acids isolated from tumor cells revealed some possible peculiarities of their structural organization, namely, the appearance of unusual sugar and base conformations, modification of the phosphate backbone, redistribution of the H-bond net, and so forth. This method enhanced a set of the bands, which is impossible to observe in conventional IR geometry. The SEIRA spectra of the RNA from tumor cells showed more sensitivity to the grade of tumor malignancy than the spectra of the DNA. After application of the anticancer drug doxorubicin to sensitive and resistant strains, the DNA isolated from these strains had different spectral features, especially in the region of the phosphate I and II bands. As induced by anticancer drugs, the conformational changes in the DNA from resistant and sensitive cancer strains could be characterized with different levels of structure disordering. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 470–486, 2002Keywords
This publication has 21 references indexed in Scilit:
- FTIR spectroscopy studies of nucleic acid damageTalanta, 2000
- Surface enhanced infrared absorption observed with attenuated total reflection (ATR-SEIRA): modeling the optical responseAnalytical and Bioanalytical Chemistry, 1998
- Surface enhanced infrared absorption spectroscopy (SEIRA) using external reflection on low-cost substratesAnalytical and Bioanalytical Chemistry, 1998
- Surface enhanced FTIR spectroscopy on membranesAnalytical and Bioanalytical Chemistry, 1998
- Infrared spectral models demonstrate that exposure to environmental chemicals leads to new forms of DNAProceedings of the National Academy of Sciences of the United States of America, 1997
- Potential-Dependent Reorientation of Water Molecules at an Electrode/Electrolyte Interface Studied by Surface-Enhanced Infrared Absorption SpectroscopyThe Journal of Physical Chemistry, 1996
- The etiology and prediction of breast cancer. Fourier transform-infrared spectroscopy reveals progressive alterations in breast DNA leading to a cancer-like phenotype in a high proportion of normal womenCancer, 1995
- Surface-enhanced infrared absorption of p-nitrobenzoic acid deposited on silver island films: contributions of electromagnetic and chemical mechanismsThe Journal of Physical Chemistry, 1991
- Surface Enhanced Raman ScatteringPublished by Springer Science and Business Media LLC ,1982
- Enhancement of the Infrared Absorption from Molecular Monolayers with Thin Metal OverlayersPhysical Review Letters, 1980