Detection of morphological markers of vulnerable atherosclerotic plaque using multimodal spectroscopy
Open Access
- 1 January 2006
- journal article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 11 (2), 021007-021007-9
- https://doi.org/10.1117/1.2187943
Abstract
Vulnerable plaques, which are responsible for most acute ischemic events, are presently invisible to x-ray angiography. Their primary morphological features include a thin or ulcerated fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We present evidence that multimodal spectroscopy (MMS), a novel method that combines diffuse reflectance spectroscopy (DRS), intrinsic fluorescence spectroscopy (IFS), and Raman spectroscopy (RS), can detect these markers of plaque vulnerability. To test this concept, we perform an MMS feasibility study on 17 human carotid artery specimens. Following the acquisition of spectra, each specimen is histologically evaluated. Two parameters from DRS, hemoglobin concentration and a scattering parameter, are used to detect intraplaque hemorrhage and foam cells; an IFS parameter that relates to the amount of collagen in the topmost layers of the tissue is used to detect the presence of a thin fibrous cap; and an RS parameter related to the amount of cholesterol and necrotic material is used to detect necrotic core. Taken together, these spectral parameters can generally identify the vulnerable plaques. The results indicate that MMS provides depth-sensitive and complementary morphological information about plaque composition. A prospective in vivo study will be conducted to validate these findings.Keywords
This publication has 41 references indexed in Scilit:
- Photobleaching of Arterial Fluorescent Compounds: Characterization of Elastin, Collagen and Cholesterol Time-resolved Spectra during Prolonged Ultraviolet IrradiationPhotochemistry and Photobiology, 1999
- Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantomsApplied Optics, 1997
- Analytical model for extracting intrinsic fluorescence in turbid mediaApplied Optics, 1993
- β‐Carotene enhances plaque detection by fluorescence attenuation in an atherosclerotic rabbit modelLasers in Surgery and Medicine, 1993
- Detection of calcified atherosclerotic plaque by laser-induced plasma emissionLasers in Surgery and Medicine, 1992
- 476 nm Excited laser-induced fluorescence spectroscopy of human coronary arteries: Applications in cardiologyAmerican Heart Journal, 1991
- Laser induced fluorescence spectroscopy of normal and atherosclerotic human aorta using 306–310 nm excitationLasers in Surgery and Medicine, 1990
- Argon ion laser-excited autofluorescence in normal and atherosclerotic aorta and coronary arteries: Morphologic studiesAmerican Heart Journal, 1989
- Spectral diagnosis of atherosclerosis using an optical fiber laser catheterAmerican Heart Journal, 1989
- Statistical Comparison of Multiple Analytic Procedures: Application to Clinical ChemistryTechnometrics, 1979