Preparation and characterization of a monoclonal antibody specific for the β‐agonist clenbuterol
- 1 March 1996
- journal article
- research article
- Published by Informa UK Limited in Food and Agricultural Immunology
- Vol. 8 (1), 3-10
- https://doi.org/10.1080/09540109609354898
Abstract
A monoclonal antibody was obtained from a Balb/c mouse immunized with a conjugate prepared by the direct coupling of diazotized clenbuterol to bovine serum albumin. The antibody, characterized by γ1,κ isotype and an affinity constant of 3.5 × 108 l mol‐1, is highly specific as its cross‐reactivity to structurally related molecules is less than 1%. A competitive ELISA for the detection of clenbuterol in horse and human urine was developed using this antibody.Keywords
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