A Trojan horse mechanism of bacterial pathogenesis against nematodes
Open Access
- 23 August 2010
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 107 (38), 16631-16636
- https://doi.org/10.1073/pnas.1007276107
Abstract
Understanding the mechanisms of host–pathogen interaction can provide crucial information for successfully manipulating their relationships. Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive host for studying microbial pathogenesis. Here we report a “Trojan horse” mechanism of bacterial pathogenesis against nematodes. We show that the bacterium Bacillus nematocida B16 lures nematodes by emitting potent volatile organic compounds that are much more attractive to worms than those from ordinary dietary bacteria. Seventeen B. nematocida-attractant volatile organic compounds are identified, and seven are individually confirmed to lure nematodes. Once the bacteria enter the intestine of nematodes, they secrete two proteases with broad substrate ranges but preferentially target essential intestinal proteins, leading to nematode death. This Trojan horse pattern of bacterium–nematode interaction enriches our understanding of microbial pathogenesis.Keywords
This publication has 43 references indexed in Scilit:
- Enterobacter sakazakii Invasion in Human Intestinal Caco-2 Cells Requires the Host Cell Cytoskeleton and Is Enhanced by Disruption of Tight JunctionInfection and Immunity, 2008
- Roles forDrosophila melanogasterMyosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial PathogenPseudomonas entomophilaMolecular Biology of the Cell, 2007
- Detection and avoidance of a natural product from the pathogenic bacterium Serratia marcescens by Caenorhabditis elegansProceedings of the National Academy of Sciences of the United States of America, 2007
- Caenorhabditis elegans Senses Bacterial AutoinducersApplied and Environmental Microbiology, 2006
- VHA‐8, the E subunit of V‐ATPase, is essential for pH homeostasis and larval development in C. elegansFEBS Letters, 2006
- Pathogenic bacteria induce aversive olfactory learning in Caenorhabditis elegansNature, 2005
- Effects of Enterococcus faecalis fsr Genes on Production of Gelatinase and a Serine Protease and VirulenceInfection and Immunity, 2000
- Cloning and characterisation of a gene encoding a cuticle-degrading protease from the insect pathogenic fungus Metarhizium anisopliaeGene, 1995
- Immunocytochemical localization of a 32-kDa protease from the nematophagous fungusVerticillium suchlasporium in infected nematode eggsExperimental Mycology, 1992
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970