Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator
Open Access
- 26 May 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (21), 8617-8622
- https://doi.org/10.1073/pnas.0900592106
Abstract
In bacteria, many “atypical” response regulators (ARRs) lack the conserved residues important for phosphorylation by which typical response regulators switch their output response, suggesting the existence of alternative regulatory mechanisms. However, such mechanisms have not been established. JadR1, an OmpR-type ARR of Streptomyces venezuelae, appears to activate the transcription of jadomycin B (JdB) biosynthetic genes while repressing its own gene. JadR1 activities were inhibited in cells induced to produce JdB, which was found to bind directly to the N-terminal receiver domain of JadR1, causing JadR1 to dissociate from target promoters. The activity of a NarL-type ARR, RedZ, that regulates production of another antibiotic was likewise modulated by the end product (undecylprodigisines), implying that end-product-mediated control of antibiotic pathway-specific ARRs may be widespread. These results could prove relevant to knowledge-based improvements in yield of commercially important antibiotics.This publication has 40 references indexed in Scilit:
- Rewiring the Specificity of Two-Component Signal Transduction SystemsCell, 2008
- An atypical receiver domain controls the dynamic polar localization of the Myxococcus xanthus social motility protein FrzSMolecular Microbiology, 2007
- An Unusual Response Regulator Influences Sporulation at Early and Late Stages inStreptomyces coelicolorJournal of Bacteriology, 2007
- The Orphan Response Regulator HP1021 of Helicobacter pylori Regulates Transcription of a Gene Cluster Presumably Involved in Acetone MetabolismJournal of Bacteriology, 2007
- Biosynthesis of γ-butyrolactone autoregulators that switch on secondary metabolism and morphological development in StreptomycesProceedings of the National Academy of Sciences of the United States of America, 2007
- Structural Classification of Bacterial Response Regulators: Diversity of Output Domains and Domain CombinationsJournal of Bacteriology, 2006
- Pivotal Roles for the Receiver Domain in the Mechanism of Action of the Response Regulator RamR of Streptomyces coelicolorJournal of Molecular Biology, 2005
- Regulation of Iron Transport inStreptococcus pneumoniaeby RitR, an Orphan Response RegulatorJournal of Bacteriology, 2004
- AglZ Is a Filament-Forming Coiled-Coil Protein Required for Adventurous Gliding Motility ofMyxococcus xanthusJournal of Bacteriology, 2004
- Control of growth, secondary metabolism and sporulation in Streptomyces venezuelae ISP5230 by jadW 1, a member of the afsA family of γ-butyrolactone regulatory genesMicrobiology, 2003