In vitro assay of allergenic properties of ragweed pollen antigens
- 30 September 1966
- journal article
- Published by Elsevier BV in Journal of Allergy
- Vol. 38 (3), 174-182
- https://doi.org/10.1016/0021-8707(66)90040-2
Abstract
An in vitro reaction system, based on the release of histamine from isolated leukocytes of allergic donors, has been used to assay the allergenic activity of ragweed pollen antigens. Antigen E was the most potent allergen in tests with leukocyte suspensions of different individuals. The specific activity of other antigens, relative to E, ranged from about 0.5 to 47 per cent. The leukocytes of different individuals vary in their pattern of sensitivity to the several antigens in ragweed pollen. Data are given to show that the sensitivity of this in vitro bioassay procedure exceeds that of the skin test by about twenty times.Keywords
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