Cross-Link-Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications
- 9 November 2020
- journal article
- research article
- Published by Wiley in Angewandte Chemie-International Edition
- Vol. 59 (46), 20552-20560
- https://doi.org/10.1002/anie.202008083
Abstract
Most NIR-IIb fluorophores are nanoparticle-based probes with long retention (approximate to 1 month or longer) in the body. Here, we applied a novel cross-linked coating to functionalize core/shell lead sulfide/cadmium sulfide quantum dots (PbS/CdS QDs) emitting at approximate to 1600 nm. The coating was comprised of an amphiphilic polymer followed by three crosslinked amphiphilic polymeric layers (P(3)coating), imparting high biocompatibility and >90 % excretion of QDs within 2 weeks of intravenous administration. The P-3-QDs were conjugated to an engineered anti-CD8 diabody (Cys-diabody) for in vivo molecular imaging of CD8+ cytotoxic T lymphocytes (CTLs) in response to anti-PD-L1 therapy. Two-plex molecular imaging in combination with down-conversion Er nanoparticles (ErNPs) was performed for real-time in vivo monitoring of PD-L1 positive tumor cells and CTLs with cellular resolution by non-invasive NIR-IIb light sheet microscopy. Imaging of angiogenesis in the tumor microenvironment and of lymph nodes deep in the body with a signal-to-background ratio of up to approximate to 170 was also achieved using P-3-QDs.Funding Information
- National Institutes of Health (DP1-NS-105737)
This publication has 72 references indexed in Scilit:
- Improved functionalization of oleic acid-coated iron oxide nanoparticles for biomedical applicationsJournal of Nanoparticle Research, 2012
- Ag2S Quantum Dot: A Bright and Biocompatible Fluorescent Nanoprobe in the Second Near-Infrared WindowACS Nano, 2012
- In vivo near-infrared fluorescence imaging of CD105 expression during tumor angiogenesisEuropean Journal of Nuclear Medicine and Molecular Imaging, 2011
- A route to brightly fluorescent carbon nanotubes for near-infrared imaging in miceNature Nanotechnology, 2009
- Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumoursCardiovascular Research, 2009
- Nano-graphene oxide for cellular imaging and drug deliveryNano Research, 2008
- Lymph node mapping in the mouseJournal of Immunological Methods, 2008
- Circulation and long-term fate of functionalized, biocompatible single-walled carbon nanotubes in mice probed by Raman spectroscopyProceedings of the National Academy of Sciences of the United States of America, 2008
- Renal clearance of quantum dotsNature Biotechnology, 2007
- The Near Infrared Absorption Spectrum of Liquid WaterJournal of the Optical Society of America, 1951