Dynamics of sperm DNA fragmentation in patients carrying structurally rearranged chromosomes
- 28 April 2011
- journal article
- Published by Wiley in International Journal of Andrology
- Vol. 34 (6pt2), e546-e553
- https://doi.org/10.1111/j.1365-2605.2011.01153.x
Abstract
This investigation was conducted to assess the baseline level of sperm DNA fragmentation (SDF) in a cohort of patients presenting chromosomal rearrangements (nine reciprocal translocations and two inversions). In a separate experiment, a dynamic analysis to calculate the rate of SDF (rSDF), after a varying period of sperm storage (0 h, 1 h, 4 h, 8 h and 24 h) at 37 °C, was performed. Results were compared with eight fertile donors. Different experimental approaches to assess SDF, such as terminal transferase dUTP nick-end labelling (TUNEL), sperm chromatin structure assay (SCSA) and sperm chromatin dispersion test (SCDt), were used. No differences for the baseline level of SDF were found. Carriers of reorganized genomes showed statistically higher levels of SDF than did control donors (p = 0.025 for TUNEL; p = 0.022 for SCSA; p = 0.014 for SCDt). However, 54.5% (6/11) of the patients presented values similar to those of control donors. There was no significant difference in rSDF (p = 0.34). Nevertheless, the results suggest that a high variability for SDF and rSDF exists in these patients. Routine analysis of SDF and rSDF should be considered in patients presenting rearranged genomes to determine fertility status for assisted reproductive techniques (ART) purposes.Keywords
This publication has 39 references indexed in Scilit:
- Apoptosis and meiotic segregation in ejaculated sperm from Robertsonian translocation carrier patientsHuman Reproduction, 2010
- On the possible origins of DNA damage in human spermatozoaMolecular Human Reproduction, 2009
- The aetiology of sperm protamine abnormalities and their potential impact on the sperm epigenomeInternational Journal of Andrology, 2008
- Alkali-labile sites in sperm cells from Sus and Ovis speciesInternational Journal of Andrology, 2008
- REVIEW ARTICLE: Clinical Relevance of Oxidative Stress in Male Factor Infertility: An UpdateAmerican Journal of Reproductive Immunology, 2007
- Altered protamine expression and diminished spermatogenesis: what is the link?Human Reproduction Update, 2007
- Effect of cryopreservation on sperm apoptotic deoxyribonucleic acid fragmentation in patients with oligozoospermiaFertility and Sterility, 2006
- Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human SpermJournal of Andrology, 2006
- Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patientsHuman Reproduction, 2005
- Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoaHuman Reproduction, 2000