Dynein and kinesin regulate stress-granule and P-body dynamics
Open Access
- 1 November 2009
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 122 (21), 3973-3982
- https://doi.org/10.1242/jcs.051383
Abstract
Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs are constitutive and are further induced by stress. Both foci are highly dynamic, with messenger ribonucleoproteins (mRNPs) and proteins rapidly shuttling in and out. Here, we show that impairment of retrograde transport by knockdown of mammalian dynein heavy chain 1 (DHC1) or bicaudal D1 (BicD1) inhibits SG formation and PB growth upon stress, without affecting protein-synthesis blockage. Conversely, impairment of anterograde transport by knockdown of kinesin-1 heavy chain (KIF5B) or kinesin light chain 1 (KLC1) delayed SG dissolution. Strikingly, SG dissolution is not required to restore translation. Simultaneous knockdown of dynein and kinesin reverted the effect of single knockdowns on both SGs and PBs, suggesting that a balance between opposing movements driven by these molecular motors governs foci formation and dissolution. Finally, we found that regulation of SG dynamics by dynein and kinesin is conserved in Drosophila.Keywords
This publication has 51 references indexed in Scilit:
- Dynein motor contributes to stress granule dynamics in primary neuronsNeuroscience, 2009
- Kinesin Transports RNA: Isolation and Characterization of an RNA-Transporting GranuleNeuron, 2004
- Disruption of microtubules inhibits cytoplasmic ribonucleoprotein stress granule formationExperimental Cell Research, 2003
- RETRACTED: The RasGAP-associated endoribonuclease G3BP assembles stress granulesThe Journal of cell biology, 2003
- Dynactin is required for bidirectional organelle transportThe Journal of cell biology, 2003
- Kinesin light chain-independent function of theKinesin heavy chainin cytoplasmic streaming and posterior localisation in theDrosophilaoocyteDevelopment, 2002
- Bicaudal-D regulates COPI-independent Golgi–ER transport by recruiting the dynein–dynactin motor complexNature, 2002
- Mammalian Golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexesThe EMBO Journal, 2001
- Regulation of kinesin: implications for neuronal development.Developmental Neuroscience, 2001
- Dynamic Shuttling of Tia-1 Accompanies the Recruitment of mRNA to Mammalian Stress GranulesThe Journal of cell biology, 2000