Alginic acid has anti‐anaphylactic effects and inhibits inflammatory cytokine expression via suppression of nuclear factor‐κB activation

Abstract

Background Alginic acid is comprised of complex polymerized polysaccharides, and can be chemically extracted from seaweed. Alginic acid has an inhibitory effect on histamine release, but its molecular mechanisms are not well understood. Objective To investigate the effect of alginic acid on the mast cell‐mediated anaphylactic and inflammatory reaction using in vivo and in vitro models and elucidate its molecular mechanisms. Materials and method The effect of alginic acid on an allergy model was analysed by anaphylaxis, a histidine decarboxylase (HDC) assay, and a histamine assay. Cytokine production was analysed by means of ELISA. Cytokine expression was analysed by means of RT‐PCR, and Western blotting. Transcription factor activity was analysed by a luciferase assay and a transcription factor‐enzyme linked immunoassay. Results Alginic acid dose dependently inhibited compound 48/80‐induced systemic anaphylaxis with doses of 0.25–1 g/kg 1 h (Pn=6) and significantly inhibited passive cutaneous anaphylaxis by 54.8%. Alginic acid (0.01–1 μg/mL) inhibited histamine release from serum and peritoneal mast cells (P/Rel A protein increased in the nucleus, whereas the level of NF‐κB/Rel A in the nucleus was decreased by alginic acid treatment. In addition, alginic acid (0.01 μg/mL) decreased the PMA+A23187‐induced luciferase activity and DNA‐binding activity. Conclusion The present results indicate that alginic acid has potent anti‐anaphylactic and anti‐inflammatory properties.