Directional molecular sliding movement in peptide hydrogels accelerates cell proliferation
Open Access
- 7 February 2020
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Science
- Vol. 11 (5), 1383-1393
- https://doi.org/10.1039/c9sc05808g
Abstract
Adjusting the mechanical cues generated in cellular microenvironments is important for manipulating cell behaviour. Here we report on mechanically dynamic hydrogels undergoing directional domain sliding motion and investigate the effect of the well-defined mechanical motion on accelerating cell proliferation. The mechanically dynamic hydrogels were prepared via self-assembly of an amphiphilic peptide consisting of two alternating polar and nonpolar domains cross-linked by disulfide bonds at a nonsymmetrical position. The cross-linked peptide assembled into entangled nanofibers driven by the hydrophobic collapse involving a partial-length sequence due to the covalent constraint. Reduction of the disulfide bonds led to formation of non-equilibrated peptide bilayers, which underwent directional domain sliding motion along each promoted by the thermodynamically favourable transition from the partial to full hydrophobic collapse. The mechanical cues resulting from the directional domain sliding motion within the mechanically dynamic hydrogels accelerated cell proliferation when incubating cells on the hydrogel, compared to the thermodynamically static counterparts, via a mechanotransduction mechanism as supported by the facilitated translocation of yes-associated proteins into the nucleus of the cells. Our finding demonstrates the great potential of mechanically dynamic hydrogels as new-generation biomimetic extracellular matrices in tissue engineering and regeneration.Funding Information
- National Natural Science Foundation of China (21774065, 31600768, 51933006, 21620102005)
- Fundamental Research Funds for the Central Universities (ZB19100123, 63186058)
This publication has 73 references indexed in Scilit:
- Multi-hierarchical self-assembly of a collagen mimetic peptide from triple helix to nanofibre and hydrogelNature Chemistry, 2011
- The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogelsBiomaterials, 2011
- De novo designed peptides for biological applicationsChemical Society Reviews, 2011
- Multi-component extracellular matrices based on peptide self-assemblyChemical Society Reviews, 2010
- Tuning Supramolecular Rigidity of Peptide Fibers through Molecular StructureJournal of the American Chemical Society, 2010
- Rational design and application of responsive α-helical peptide hydrogelsNature Materials, 2009
- Growth Factors, Matrices, and Forces Combine and Control Stem CellsScience, 2009
- Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical PropertiesScience, 2009
- Local force and geometry sensing regulate cell functionsNature Reviews Molecular Cell Biology, 2006
- The Hippo Signaling Pathway Coordinately Regulates Cell Proliferation and Apoptosis by Inactivating Yorkie, the Drosophila Homolog of YAPCell, 2005