Microanalysis of Ginseng Saponins by Ion Chromatography with Pulsed Amperometric Detection

Abstract

Analysis of ginseng saponins by ion chromatographic separation with pulsed amperometric detection(IOPAD) was examined. Ginseng saponins were separated on Carbopac PA1 or AS4A anion exchange column with 1M NaOH as mobile phase. The measuring potential(El), oxidation potential(E2), and reduction potential(E3) were 0.0V, +0.6V, and -0.8V, respectively. The dynamic linear range was over three orders and the limit of detection of ginsenoside Re was 0.8 pmol(S/N=2).