Spatiotemporal characterization of rhythmic activity in rat spinal cord slice cultures
- 1 July 2001
- journal article
- research article
- Published by Wiley in European Journal of Neuroscience
- Vol. 14 (2), 179-190
- https://doi.org/10.1046/j.0953-816x.2001.01635.x
Abstract
Rat spinal networks generate a spontaneous rhythmic output directed to motoneurons under conditions of increased excitation or of disinhibition. It is not known whether these differently induced rhythms are produced by a common rhythm generator. To investigate the generation and the propagation of rhythmic activity in spinal networks, recordings need to be made from many neurons simultaneously. Therefore extracellular multisite recording was performed in slice cultures of embryonic rat spinal cords grown on multielectrode arrays. In these organotypic cultures most of the spontaneous neural activity was nearly synchronized. Waves of activity spread from a source to most of the network within 35–85 ms and died out after a further 30–400 ms. Such activity waves induced the contraction of cocultured muscle fibres. Several activity waves could be grouped into aperiodic bursts. Disinhibition with bicuculline and strychnine or increased excitability with high K+ or low Mg2+ solutions could induce periodic bursting with bursts consisting of one or several activity waves. Whilst the duration and period of activity waves were similar for all protocols, the duration and period of bursts were longer during disinhibition than during increased excitation. The sources of bursting activity were mainly situated ventrally on both sides of the central fissure. The pathways of network recruitment from one source were variable between bursts, but they showed on average no systematic differences between the protocols. These spatiotemporal similarities under conditions of increased excitation and of disinhibition suggest a common spinal network for both types of rhythmic activity.Keywords
This publication has 40 references indexed in Scilit:
- Generation of rhythmic patterns of activity by ventral interneurones in rat organotypic spinal slice cultureThe Journal of Physiology, 1999
- Distribution of Central Pattern Generators for Rhythmic Motor Outputs in the Spinal Cord of Limbed VertebratesaAnnals of the New York Academy of Sciences, 1998
- Network bursting by organotypic spinal slice cultures in the presence of bicuculline and/or strychnine is developmentally regulatedEuropean Journal of Neuroscience, 1998
- Intrinsic function of a neuronal network — a vertebrate central pattern generatorBrain Research Reviews, 1998
- Contribution of NMDA and non–NMDA glutamate receptors to locomotor pattern generation in the neonatal rat spinal cordProceedings Of The Royal Society B-Biological Sciences, 1997
- Potassium and calcium channel dependence of bursting in cultured neuronal networksBrain Research, 1994
- An Organotypic Spinal Cord‐Dorsal Root Ganglion‐Skeletal Muscle Coculture of Embryonic Rat. II. Functional Evidence for the Formation of Spinal Reflex Arcs In VitroEuropean Journal of Neuroscience, 1991
- An Organotypic Spinal Cord‐Dorsal Root Ganglion‐Skeletal Muscle Coculture of Embryonic Rat. I. The Morphological Correlates of the Spinal Reflex ArcEuropean Journal of Neuroscience, 1991
- N-Methyl-d,l-aspartate-induced locomotor activity in a spinal cord-indlimb muscles preparation of the newborn rat studied in vitroNeuroscience Letters, 1987
- ORGANOTYPIC MONOLAYER-CULTURES OF NERVOUS-TISSUEJournal of Neuroscience Methods, 1980