Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SS
Open Access
- 1 January 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 13 (1), 231
- https://doi.org/10.1186/1471-2180-13-231
Abstract
Cis-2-dodecenoic acid (BDSF) is well known for its important functions in intraspecies signaling in Burkholderia cenocepacia. Previous work has also established an important role of BDSF in interspecies and inter-kingdom communications. It was identified that BDSF modulates virulence of Pseudomonas aeruginosa. However, how BDSF interferes with virulence of P. aeruginosa is still not clear. We report here that BDSF mediates the cross-talk between B. cenocepacia and P. aeruginosa through interference with quorum sensing (QS) systems and type III secretion system (T3SS) of P. aeruginosa. Bioassay results revealed that exogenous addition of BDSF not only reduced the transcriptional expression of the regulator encoding gene of QS systems, i.e., lasR, pqsR, and rhlR, but also simultaneously decreased the production of QS signals including 3-oxo-C12-HSL, Pseudomonas quinolone signal (PQS) and C4-HSL, consequently resulting in the down-regulation of biofilm formation and virulence factor production of P. aeruginosa. Furthermore, BDSF and some of its derivatives are also capable of inhibiting T3SS of P. aeruginosa at a micromolar level. Treatment with BDSF obviously reduced the virulence of P. aeruginosa in both HeLa cell and zebrafish infection models. These results depict that BDSF modulates virulence of P. aeruginosa through interference with QS systems and T3SS.Keywords
This publication has 63 references indexed in Scilit:
- Cis-2-dodecenoic acid quorum sensing system modulates N-acyl homoserine lactone production through RpfR and cyclic di-GMP turnover in Burkholderia cenocepaciaBMC Microbiology, 2013
- Pseudomonas aeruginosa Cytotoxicity Is Attenuated at High Cell Density and Associated with the Accumulation of Phenylacetic AcidPLOS ONE, 2013
- Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnoverProceedings of the National Academy of Sciences of the United States of America, 2012
- Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosaThe ISME Journal, 2011
- Malyngolide from the cyanobacterium Lyngbya majuscula interferes with quorum sensing circuitryEnvironmental Microbiology Reports, 2010
- Transcriptional regulation of the Pseudomonas aeruginosa type III secretion systemMolecular Microbiology, 2006
- Quorum sensing and signal interference: diverse implicationsMolecular Microbiology, 2004
- Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coliProceedings of the National Academy of Sciences of the United States of America, 1999
- A hierarchical quorum‐sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary‐phase sigma factor RpoSMolecular Microbiology, 1996
- A modified procedure for the preparation of insoluble chromogenic substrates for the determination of proteolytic activityJournal of Biochemical and Biophysical Methods, 1987