Seric and secretory antibodies reactive to α, β and γ intimins ofEscherichia coliin healthy Brazilian adults
Open Access
- 1 October 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 57 (1), 32-39
- https://doi.org/10.1111/j.1574-695x.2009.00576.x
Abstract
Intimin is essential for attaching and effacing lesions by pathogens such as enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC), and the antigenic polymorphism of intimin determines distinct subtypes. Our aim was to investigate the presence of immunoglobulin G (IgG) and IgA antibodies reactive to α, β and γ intimins in serum and colostrum from healthy Brazilian adults. We found seric IgG and secretory IgA antibodies reactive to conserved and variable regions of α, β and γ intimins and a positive correlation between the concentrations of these antibodies in both serum and colostrum that suggested cross reactivity among anti-intimin antibodies, as was confirmed by immunoblotting and absorption. The concentrations of anti-conserved region antibodies were higher than those of variable region antibodies. The presence of antibodies reactive to EHEC antigens could result from contact with EPEC or with other bacteria of the environment even though this bacterium is not frequent in Brazil, and suggests possible protection against EHEC.Keywords
This publication has 25 references indexed in Scilit:
- Typical Enteroaggregative and Atypical Enteropathogenic Types of Escherichia coli Are the Most Prevalent Diarrhea-Associated Pathotypes among Brazilian ChildrenJournal of Clinical Microbiology, 2007
- Functional studies of intimin in vivo and ex vivo: implications for host specificity and tissue tropismMicrobiology, 2007
- Phenotypic characteristics, virulence profile and genetic relatedness of O157 Shiga toxin-producing Escherichia coli isolated in Brazil and other Latin American countriesFEMS Microbiology Letters, 2006
- Emerging Enterohaemorrhagic Escherichia coli, Causes and Effects of the Rise of a Human PathogenJournal of Veterinary Medicine, Series B, 2006
- Secretory immunoglobulin A obtained from pooled human colostrum and milk for oral passive immunizationPediatric Allergy and Immunology, 2005
- Emerging EnteropathogenicEscherichia coliStrains?Emerging Infectious Diseases, 2004
- Pathogenic Escherichia coliNature Reviews Microbiology, 2004
- Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosisBrazilian Journal of Microbiology, 2003
- Antibody response of patients infected with verocytotoxin-producing Escherichia coli to protein antigens encoded on the LEE locusJournal of Medical Microbiology, 2000
- The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model.JCI Insight, 1993