Triazole-Resistant Aspergillus fumigatus from Fungicide-Experienced Soils in Naivasha Subcounty and Nairobi County, Kenya
Open Access
- 26 June 2018
- journal article
- research article
- Published by Hindawi Limited in International Journal of Microbiology
- Vol. 2018, 1-6
- https://doi.org/10.1155/2018/7147938
Abstract
The mainstay in prevention and treatment of aspergillosis is the use triazole drugs. In Kenya, the use of agricultural azole is one of the predisposing factors in development of resistance. One hundred fifty-six (156) experienced soils were collected from agricultural farms and cultured on Sabouraud DextroseAagar. The study isolated 48 yielded Aspergillus fumigatus and 2 A. flavus. All the isolates were subjected to antifungal susceptibility testing against three triazoles: posaconazole, voriconazole, and itraconazole. Out of the isolates, 3 had MIC of 32 and 1 had MIC of 16 against itraconazole, and 1 isolate had MIC of 32 against posaconazole. CYP51A gene was sequenced, and TR34/L98H mutation was identified. Triazole resistance existing in Kenya calls for rational use of azole-based fungicides in agriculture over concerns of emerging antifungal resistance in clinical practice.Keywords
Funding Information
- Kenya Medical Research Institute (INNOV/IGR/019/2)
This publication has 36 references indexed in Scilit:
- Clonal Expansion and Emergence of Environmental Multiple-Triazole-Resistant Aspergillus fumigatus Strains Carrying the TR34/L98H Mutations in the cyp51A Gene in IndiaPLOS ONE, 2012
- Triazole Fungicides Can Induce Cross-Resistance to Medical Triazoles in Aspergillus fumigatusPLOS ONE, 2012
- High Prevalence of Azole-Resistant Aspergillus fumigatus in Adults with Cystic Fibrosis Exposed to ItraconazoleAntimicrobial Agents and Chemotherapy, 2012
- Azole Resistance in Aspergillus fumigatus Isolates from the ARTEMIS Global Surveillance Study Is Primarily Due to the TR/L98H Mutation in the cyp51A GeneAntimicrobial Agents and Chemotherapy, 2011
- Regulatory Circuitry Governing Fungal Development, Drug Resistance, and DiseaseMicrobiology and Molecular Biology Reviews, 2011
- Aspergillus Species and Other Molds in Respiratory Samples from Patients with Cystic Fibrosis: a Laboratory-Based Study with Focus on Aspergillus fumigatus Azole ResistanceJournal of Clinical Microbiology, 2011
- Environmental Study of Azole-Resistant Aspergillus fumigatus and Other Aspergilli in Austria, Denmark, and SpainAntimicrobial Agents and Chemotherapy, 2010
- Possible Environmental Origin of Resistance of Aspergillus fumigatus to Medical TriazolesApplied and Environmental Microbiology, 2009
- Emergence of Azole Resistance in Aspergillus fumigatus and Spread of a Single Resistance MechanismPLoS Medicine, 2008
- A New Aspergillus fumigatus Resistance Mechanism Conferring In Vitro Cross-Resistance to Azole Antifungals Involves a Combination of cyp51A AlterationsAntimicrobial Agents and Chemotherapy, 2007