Chemical profile, thermodynamic stability and fungicidal activity of the nanoemulsion incorporated with essential oil and hydroalcoholic extract of Syzygium aromaticum (L.) Merr. & LM.Perry

Abstract

.This work aimed to evaluate the fungicidal activity of nanoemulsions of hydroalcoholic extract and essential oil of sprouts of Syzygium aromaticum. For the extraction of essential oil, the hydrodistillation technique was used. To obtain the hydroalcoholic extract, the process of maceration with solvent extract methanol P.A 70% (v / v) was performed. Nanoemulsions were obtained using the low-energy phase inversion method. For the fungicidal activity, the Broth Dilution and Agar Seeding assay was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM). The action of the biotechnological product was tested against three strains of fungi Aspergillus niger, Colletotrichum gloeosporioides, Penicillium chrysogenum. For antifungal action of the essential oil nanoemulsion, the MIC obtained was 25 µg mL-1, 25 µg mL-1 and 50 µg mL-1against A. niger, C. gloeosporioides and P. chrysogenum, respectively, for the hydroalcoholic extract nanoemulsion the result was 50 µg mL-1, 50 µg mL-1 and 100 µg mL-1 for A. niger, C. gloeosporioides and P. chrysogenum, respectively. Finally, this study points to the nanoemulsion of S. aromaticum as an alternative fungicide in the control and combat of pathogenic fungi.