Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments
- 1 October 1998
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 30 (2), 393-404
- https://doi.org/10.1046/j.1365-2958.1998.01075.x
Abstract
The Gram‐positive bacterium Staphylococcus aureus infects diverse tissues and causes a wide spectrum of diseases, suggesting that it possesses a repertoire of distinct molecular mechanisms promoting bacterial survival in disparate in vivo environments. Signature‐tag transposon mutagenesis screening of a 1520‐member library identified numerous S. aureus genetic loci affecting growth and survival in four complementary animal infection models including mouse abscess, bacteraemia and wound and rabbit endocarditis. Of a total of 237 in vivo attenuated mutants identified by the murine models, less than 10% showed attenuation in all three models, emphasizing the advantage of screening in diverse disease environments. The largest gene class identified by these analyses encoded peptide and amino acid transporters, some of which were important for S. aureus survival in all animal infection models tested. The identification of staphylococcal loci affecting growth, persistence and virulence in multiple tissue environments provides insight into the complexities of human infection and on the molecular mechanisms that could be targeted by new antibacterial therapies.Keywords
This publication has 46 references indexed in Scilit:
- Analysis of non-polar deletion mutations in the genes of the spo0K (opp) operon of Bacillus subtilisFEMS Microbiology Letters, 1997
- Beyond vancomycin: new therapies to meet the challenge of glycopeptide resistanceTrends in Microbiology, 1997
- Emerging Resistance to Antimicrobial Agents in Gram-Positive BacteriaDrugs, 1996
- Simultaneous Identification of Bacterial Virulence Genes by Negative SelectionScience, 1995
- Diminished virulence of a sar-/agr- mutant of Staphylococcus aureus in the rabbit model of endocarditis.JCI Insight, 1994
- Growth cycle-induced changes in sensitivity of Staphylococcus aureus to bactericidal lipids from abscessesJournal of Medical Microbiology, 1993
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Staphylococcal and Streptococcal Pyrogenic Toxins Involved in Toxic Shock Syndrome and Related IllnessesCritical Reviews in Microbiology, 1990
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- The Tryptophan Gene Cluster of Staphylococcus aureusJournal of General Microbiology, 1970