Proteomic Approaches to Understanding Age-Related Macular Degeneration
- 1 January 2003
- book chapter
- review article
- Published by Springer Science and Business Media LLC in Advances in Experimental Medicine and Biology
- Vol. 533, 83-89
- https://doi.org/10.1007/978-1-4615-0067-4_11
Abstract
Microdissection methods have been developed for isolating drusen and Bruch's membrane from human eyes. Comparative proteomic studies of these isolates from normal and AMD donors were pursued for clues to the biochemical pathways involved in the pathogenesis of AMD. A total of 129 potential drusen proteins were identified by LC MS/MS and immunocytochemical analyses have confirmed drusen localization for ~16% of the proteins. The most common drusen proteins appear to be TIMP-3, clusterin, vitronectin and serum albumin. Western blot analysis suggests that carboxyethyl pyrrole-protein adducts derived from docosahexaenoate-containing lipids are more abundant in AMD than in normal tissues. Abnormal protein cross-links and advanced glycation end products were also observed in drusen and Bruch's membrane. Lipid oxidation products and oxidative protein modifications may be causally involved in drusen formation and Bruch's membrane thickening.Keywords
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