Ultrafast Vibrational Spectroscopy of the Flavin Chromophore
- 23 September 2006
- journal article
- Published by American Chemical Society (ACS) in The Journal of Physical Chemistry B
- Vol. 110 (41), 20107-20110
- https://doi.org/10.1021/jp0650735
Abstract
Ultrafast time-resolved infrared (TRIR) spectra of flavin adenine dinucleotide (FAD) and the anion of lumiflavin (Lf-) are described. Ground-state recovery and excited-state decay of FAD reveal a common dominant ultrafast relaxation and a minor slower component. The Lf- transient lacks a fast component. No intermediate species are observed, suggesting that the quenching mechanism is internal conversion promoted by interaction of the adenine and isoalloxazine rings in FAD. Modes are assigned, and the potential for extension of the TRIR method to photoactive proteins is discussed.Keywords
This publication has 22 references indexed in Scilit:
- Orientation of a Key Glutamine Residue in the BLUF Domain from AppA Revealed by Mutagenesis, Spectroscopy, and Quantum Chemical CalculationsJournal of the American Chemical Society, 2006
- On the Mechanism of Activation of the BLUF Domain of AppABiochemistry, 2005
- Structure of a Novel Photoreceptor, the BLUF Domain of AppA fromRhodobacter sphaeroides,Biochemistry, 2005
- From primary photochemistry to biological function in the blue-light photoreceptors PYP and AppAPublished by Springer Science and Business Media LLC ,2005
- Light-Induced Structural Changes of Apoprotein and Chromophore in the Sensor of Blue Light Using FAD (BLUF) Domain of AppA for a Signaling StateBiochemistry, 2005
- Orientational Distribution of CO before and after Photolysis of MbCO and HbCO: A Determination Using Time-Resolved Polarized Mid-IR SpectroscopyJournal of the American Chemical Society, 2004
- Development of a Broadband Picosecond Infrared Spectrometer and its Incorporation into an Existing Ultrafast Time-Resolved Resonance Raman, UV/Visible, and Fluorescence Spectroscopic ApparatusApplied Spectroscopy, 2003
- Vibrational Coupling between Amide-I and Amide-A Modes Revealed by Femtosecond Two Color Infrared SpectroscopyThe Journal of Physical Chemistry A, 2003
- Ultrafast Excited State Dynamics of Oxidized Flavins: Direct Observations of Quenching by PurinesThe Journal of Physical Chemistry A, 2000
- Ultraviolet resonance Raman spectroscopy of flavin mononucleotide and flavin-adenine dinucleotideThe Journal of Physical Chemistry, 1986