Region-specific recombination and expression are directed by portions of the Drosophila engrailed promoter.
Open Access
- 1 July 1990
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 4 (7), 1079-1093
- https://doi.org/10.1101/gad.4.7.1079
Abstract
The Drosophila engrailed gene is expressed in the cells of the posterior developmental compartments. To investigate how the engrailed gene is regulated, chimeric genes consisting of fragments of the engrailed promoter and Escherichia coli lacZ were incorporated into the Drosophila germ line by P-element-mediated recombination. Fusion constructs with 7.5 kb of 5'-flanking sequence contain sufficient information to promote expression in most of the embryonic, larval, and imaginal posterior compartments; transformants with smaller fragments of the 5' region do not. Remarkably, of 20 independent transformants with constructs containing more than 1 kb of 5'-flanking DNA, 7 integrated in or around the engrailed locus. These strains inactivate engrailed function to varying degrees, and some express lacZ with a position- and temporal-specific program that is indistinguishable from the normal engrailed gene. Presumably, in these strains, lacZ is expressed in the context of the engrailed promoter.This publication has 55 references indexed in Scilit:
- Early and late periodic patterns of even skipped expression are controlled by distinct regulatory elements that respond to different spatial cuesCell, 1989
- The molecular genetics of embryonic pattern formation in DrosophilaNature, 1988
- Isolation, structure, and expression of even-skipped: A second pair-rule gene of Drosophila containing a homeo boxCell, 1986
- The engrailed locus of drosophila: Structural analysis of an embryonic transcriptCell, 1985
- THE P FAMILY OF TRANSPOSABLE ELEMENTS IN DROSOPHILAAnnual Review of Genetics, 1983
- Localization of antigenic determinants in whole Drosophila embryosDevelopmental Biology, 1983
- Genetic Transformation of Drosophila with Transposable Element VectorsScience, 1982
- Compartments in the abdomen of Drosophila and the role of the engrailed locusDevelopmental Biology, 1981
- Anterior and posterior compartments in the proboscis of DrosophilaDevelopmental Biology, 1981
- Developmental compartmentalization in the dorsal mesothoracic disc of DrosophilaDevelopmental Biology, 1976