Determination of oleanolic acid, ursolic acid and amygdalin in the flower of Eriobotrya japonica Lindl. by HPLC
- 27 March 2007
- journal article
- research article
- Published by Wiley in Biomedical Chromatography
- Vol. 21 (7), 755-761
- https://doi.org/10.1002/bmc.817
Abstract
Simple and accurate HPLC methods were developed for the determination of oleanolic acid (OA), ursolic acid (UA) and amygdalin in loquat (Eriobotrya japonica Lindl.) flower, which is commonly used for the treatment of various diseases as a traditional Chinese medicine. HPLC assay was performed on a reversed‐phase C18 column and all three compounds were detected at 210 nm with a flow rate of 1.0 mL/min. The mobile phase consisted of methanol (A) and 0.03 mol/L phosphate buffer (pH 2.8) (B) with a ratio of 88:12 (A:B, v/v) for simultaneous detection of OA and UA, and 25:75 (A:B, v/v) for detection of amygdalin. The established methods showed good precision and accuracy with overall intra‐day and inter‐day variation of 0.99–3.55 and 1.05–4.05%, respectively, and overall recoveries of 97.37–99.32% for the three compounds. Application of these methods to determine the OA, UA and amygdalin contents in loquat flower showed that cultivar had a minor effect on the contents of all three compounds, with average amounts of 0.38–0.51 mg OA/g dry weight (DW), 2.15–2.68 mg UA/g DW and 1.23–1.56 mg amygdalin/g DW among five loquat cultivars tested. However, developmental stages and flower tissues showed significant effect on the contents of all three bioactive components. Copyright © 2007 John Wiley & Sons, Ltd.Keywords
This publication has 30 references indexed in Scilit:
- Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf in chronic bronchitis ratsLife Sciences, 2006
- Varietal differences among the anthocyanin profiles of 50 red table grape cultivars studied by high performance liquid chromatographyJournal of Chromatography A, 2005
- Variation in Ascorbic Acid and Oxalate Levels in the Fruit of Actinidia chinensis Tissues and GenotypesJournal of Agricultural and Food Chemistry, 2005
- Effect of amygdalin on the proliferation of hyperoxia-exposed type II alveolar epithelial cells isolated from premature ratJournal of Huazhong University of Science and Technology [Medical Sciences], 2004
- Anti-AIDS Agents. 30. Anti-HIV Activity of Oleanolic Acid, Pomolic Acid, and Structurally Related TriterpenoidsJournal of Natural Products, 1998
- Ursolic Acid Inhibits Aflatoxin B1-Induced Mutagenicity in a Salmonella Assay System.Biological & Pharmaceutical Bulletin, 1994
- Hypoglycemic Effects of Sesquiterpene Glycosides and Polyhydroxylated Triterpenoids ofEriobotrya japonicaPlanta Medica, 1991
- Polyhydroxylated Triterpenes fromEriobotrya japonicaPlanta Medica, 1990
- Effect ofEriobotrya japonicaon Blood Glucose Levels of Normal and Alloxan-Diabetic RabbitsPlanta Medica, 1988
- Anti-inflammatory constituents of topically applied crude drugs. I. Constituents and anti-inflammatory effect of Eriobotrya japonica Lindl.CHEMICAL & PHARMACEUTICAL BULLETIN, 1986