Antimicrobial activity of antibiotics in combination with natural flavonoids against clinical extended‐spectrum β‐lactamase (ESBL)‐producing Klebsiella pneumoniae
- 1 July 2005
- journal article
- Published by Wiley in Phytotherapy Research
- Vol. 19 (7), 612-617
- https://doi.org/10.1002/ptr.1695
Abstract
Extended‐spectrum β‐lactamases (ESBLs) are plasmid‐mediated class A enzymes commonly found in the family Enterobacteriaceae, mainly in Klebsiella pneumoniae. Flavonoids have also been reported to possess antimicrobial activity. In this study, the in vitro activities of 18 antibiotics and 12 flavonoids against 20 ESBL‐producing K. pneumoniae isolates were evaluated. All of these isolates were susceptible to imipenem and cefmetazole, but were resistant to ampicillin, ampicillin/sulbactam, aztreonam, cefazolin, cefoperazone, cefotaxime, ceftazidime, ceftriaxone, cefuroxime, piperacillin and ticarcillin. Susceptibilities to amikacin, amoxicillin/clavulanate, cefoxitin, ciprofloxacin and gentamicin were variable. Myricetin, a flavonol, inhibited ESBL‐producing K. pneumoniae isolates at a high minimum inhibitory concentration (MIC) (MIC90 value 256 mg/mL), but exhibited significant synergic activity against ESBL‐producing K. pneumoniae in separate combination with amoxicillin/clavulanate, ampicillin/sulbactam and cefoxitin. Because of the low‐toxic nature of flavonoids, the combination of antibiotics and flavonoids is a potential new strategy for developing therapies for infections caused by ESBL‐producing bacteria in the future. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 25 references indexed in Scilit:
- Relationship between ciprofloxacin resistance and extended-spectrum β-lactamase production in Escherichia coli and Klebsiella pneumoniae strainsClinical Microbiology & Infection, 2004
- Carbapenems in PediatricsTherapeutic Drug Monitoring, 2003
- Potential Therapeutic Applications of Some Antinutritional Plant Secondary MetabolitesJournal of Agricultural and Food Chemistry, 2003
- Evaluation of MicroScan ESBL confirmation panel for Enterobacteriaceae-producing, extended-spectrum β-lactamases isolated in JapanDiagnostic Microbiology and Infectious Disease, 2003
- Characterization of Clinical Isolates ofKlebsiella pneumoniaefrom 19 Laboratories Using the National Committee for Clinical Laboratory Standards Extended-Spectrum β-Lactamase Detection MethodsJournal of Clinical Microbiology, 2001
- Outer membrane protein change combined with co-existing TEM-1 and SHV-1 beta-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniaeJournal of Antimicrobial Chemotherapy, 2001
- Antibacterial Activity of Flavonoids Against Methicillin-resistant Staphylococcus aureus strainsJournal of Theoretical Biology, 2000
- The Ginkgo biloba Extract (EGb 761) Protects and Rescues Hippocampal Cells Against Nitric Oxide‐Induced ToxicityJournal of Neurochemistry, 2000
- In Vitro Evaluation of the Antimicrobial Activities of Selected LozengesJournal of Pharmaceutical Sciences, 1993
- Clinical importance of inducible beta-lactamases in gram-negative bacteriaEuropean Journal of Clinical Microbiology & Infectious Diseases, 1987