Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering
- 1 July 2014
- journal article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 20 (13-14), 1896-1907
- https://doi.org/10.1089/ten.tea.2013.0008
Abstract
Structural similarity of electrospun fibers (ESFs) to the native extracellular matrix provides great potential for the application of biofunctional ESFs in tissue engineering. This study aimed to synthesize biofunctionalized poly (L-lactide-co-glycolide) (PLGA) ESFs for investigating the potential for cardiac tissue engineering application. We developed a simple but novel strategy to incorporate adhesive peptides in PLGA ESFs. Two adhesive peptides derived from laminin, YIGSR, and RGD, were covalently conjugated to poly-L-lysine, and then mingled with PLGA solution for electrospinning. Peptides were uniformly distributed on the surface and in the interior of ESFs. PLGA ESFs incorporated with YIGSR or RGD or adsorbed with laminin significantly enhanced the adhesion of cardiomyocytes isolated from neonatal rats. Furthermore, the cells were found to adhere better on ESFs compared with flat substrates after 7 days of culture. Immunofluorescent staining of F-actin, vinculin, a-actinin, and N-cadherin indicated that cardiomyocytes adhered and formed striated α-actinin better on the laminin-coated ESFs and the YIGSR-incorporated ESFs compared with the RGD-incorporated ESFs. The expression of α-myosin heavy chain and β-tubulin on the YIGSR-incorporated ESFs was significantly higher compared with the expression level on PLGA and RGD-incorporated samples. Furthermore, the contraction of cardiomyocytes was faster and lasted longer on the laminin-coated ESFs and YIGSR-incorporated ESFs. The results suggest that aligned YIGSR-incorporated PLGA ESFs is a better candidate for the formation of cardiac patches. This study demonstrated the potential of using peptide-incorporated ESFs as designable-scaffold platform for tissue engineering.Keywords
This publication has 43 references indexed in Scilit:
- A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusionBiomaterials, 2011
- Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction modelJournal of Cellular and Molecular Medicine, 2011
- Fabrication of UV-crosslinked chitosan scaffolds with conjugation of RGD peptides for bone tissue engineeringCarbohydrate Polymers, 2011
- Electrospinning: A fascinating fiber fabrication techniqueBiotechnology Advances, 2010
- Modulation of RGD-Functionalized Polyelectrolyte Multilayer Membranes for Promoting Osteoblast FunctionJournal of Biomaterials Science, Polymer Edition, 2010
- Composite Electrospun Scaffolds for Engineering Tubular Bone GraftsTissue Engineering, Part A, 2009
- Functional electrospun nanofibrous scaffolds for biomedical applicationsAdvanced Drug Delivery Reviews, 2007
- Modulation of anisotropy in electrospun tissue-engineering scaffolds: Analysis of fiber alignment by the fast Fourier transformBiomaterials, 2006
- Cardiac extracellular matrix: a dynamic entity.American Journal of Physiology-Heart and Circulatory Physiology, 2005
- RGD and YIGSR synthetic peptides facilitate cellular adhesion identical to that of laminin and fibronectin but alter the physiology of neonatal cardiac myocytesAmerican Journal of Physiology-Cell Physiology, 2005