Bacterial Toxin-Triggered Drug Release from Gold Nanoparticle-Stabilized Liposomes for the Treatment of Bacterial Infection
- 23 February 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 133 (11), 4132-4139
- https://doi.org/10.1021/ja111110e
Abstract
We report a new approach to selectively deliver antimicrobials to the sites of bacterial infections by utilizing bacterial toxins to activate drug release from gold nanoparticle-stabilized phospholipid liposomes. The binding of chitosan-modified gold nanoparticles to the surface of liposomes can effectively prevent them from fusing with one another and from undesirable payload release in regular storage or physiological environments. However, once these protected liposomes “see” bacteria that secrete toxins, the toxins will insert into the liposome membranes and form pores, through which the encapsulated therapeutic agents are released. The released drugs subsequently impose antimicrobial effects on the toxin-secreting bacteria. Using methicillin-resistant Staphylococcus aureus (MRSA) as a model bacterium and vancomycin as a model anti-MRSA antibiotic, we demonstrate that the synthesized gold nanoparticle-stabilized liposomes can completely release the encapsulated vancomycin within 24 h in the presence of MRSA bacteria and lead to inhibition of MRSA growth as effective as an equal amount of vancomycin-loaded liposomes (without nanoparticle stabilizers) and free vancomycin. This bacterial toxin enabled drug release from nanoparticle-stabilized liposomes provides a new, safe, and effective approach for the treatment of bacterial infections. This technique can be broadly applied to treat a variety of infections caused by bacteria that secrete pore-forming toxins.Keywords
This publication has 41 references indexed in Scilit:
- Nanotechnology in Drug Delivery and Tissue Engineering: From Discovery to ApplicationsNano Letters, 2010
- Half-Antibody Functionalized Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery to Carcinoembryonic Antigen Presenting Pancreatic Cancer CellsMolecular Pharmaceutics, 2010
- Stimuli-Responsive Liposome Fusion Mediated by Gold NanoparticlesACS Nano, 2010
- The antimicrobial activity of liposomal lauric acids against Propionibacterium acnesBiomaterials, 2009
- Single Molecule Nanocontainers Made Porous Using a Bacterial ToxinJournal of the American Chemical Society, 2009
- Nanoparticle-induced surface reconstruction of phospholipid membranesProceedings of the National Academy of Sciences of the United States of America, 2008
- Biofunctionalized targeted nanoparticles for therapeutic applicationsExpert Opinion on Biological Therapy, 2008
- Cationic Nanoparticles Stabilize Zwitterionic Liposomes Better than Anionic OnesThe Journal of Physical Chemistry C, 2007
- Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolutionProceedings of the National Academy of Sciences of the United States of America, 2006
- Targeted nanoparticle-aptamer bioconjugates for cancer chemotherapy in vivoProceedings of the National Academy of Sciences of the United States of America, 2006