Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium
- 1 February 2012
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 158 (2), 390-397
- https://doi.org/10.1099/mic.0.054536-0
Abstract
The use of dental implants to treat tooth loss has increased rapidly over recent years. 'Smooth' implants showing high long-term success rates have successively been replaced by implants with rougher surfaces, designed to stimulate rapid osseointegration and promote tissue healing. If exposed in the oral cavity, rougher surfaces may promote bacterial adhesion leading to formation of microbial biofilms which can induce pen-implant inflammation. Streptococcus oralis is an early colonizer of oral surfaces and has been recovered from titanium surfaces in vivo. The purpose of this study was to examine the adherence of clinical strains of S. oralis to titanium with smooth or moderately rough surface topography and to determine the effect of a saliva- or serum-derived coating on this process. Adherence was studied using a flow-cell system with confocal laser scanning microscopy, while putative adhesins were analysed using proteomics of bacterial cell wall proteins. This showed that adherence to moderately rough surfaces was greater than to smooth surfaces. Serum did not promote binding of any of the studied S. oralis strains to titanium, whereas a saliva coating increased adherence in two of three strains tested. The higher level of adherence to the moderately rough surfaces was maintained even in the presence of a saliva coating. The S. oralis strains that bound to saliva expressed an LPXTG-linked protein which was not present in the non-adherent strain. Thus strains of S. oralis differ in their capacity to bind to saliva-coated titanium and we propose that this is due to differential expression of a novel adhesin.Keywords
This publication has 31 references indexed in Scilit:
- The road to ruin: the formation of disease‐associated oral biofilmsOral Diseases, 2010
- Oral multispecies biofilm development and the key role of cell–cell distanceNature Reviews Microbiology, 2010
- Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitroBone, 2009
- Identification of novel LPXTG-linked surface proteins from Streptococcus gordoniiMicrobiology, 2009
- Image Analysis Software Based on Color Segmentation for Characterization of Viability and Physiological Activity of BiofilmsApplied and Environmental Microbiology, 2009
- Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfacesClinical Oral Implants Research, 2008
- Acid Tolerance of Biofilm Cells of Streptococcus mutansApplied and Environmental Microbiology, 2007
- Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached BacteriaApplied and Environmental Microbiology, 2007
- The effect of saliva or serum on bacterial and Candida albicans colonization on type I collagenJournal of Oral Rehabilitation, 2006
- Mutualism versus Independence: Strategies of Mixed-Species Oral Biofilms In Vitro Using Saliva as the Sole Nutrient SourceInfection and Immunity, 2001