Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer
Top Cited Papers
- 8 July 2010
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 135 (10), 2496-2511
- https://doi.org/10.1039/c0an00204f
Abstract
This critical review evaluates progress toward viable point-of-care protein biomarker measurements for cancer detection and diagnostics. The ability to measure panels of specific, selective cancer biomarker proteins in physicians' surgeries and clinics has the potential to revolutionize cancer detection, monitoring, and therapy. The dream envisions reliable, cheap, automated, technically undemanding devices that can analyze a patient's serum or saliva in a clinical setting, allowing on-the-spot diagnosis. Existing commercial products for protein assays are reliable in laboratory settings, but have limitations for point-of-care applications. A number of ultrasensitive immunosensors and some arrays have been developed, many based on nanotechnology. Multilabel detection coupled with high capture molecule density in immunosensors and arrays seems to be capable of detecting a wide range of protein concentrations with sensitivity ranging into the sub pg mL−1 level. Multilabel arrays can be designed to detect both high and ultralow abundance proteins in the same sample. However, only a few of the newer ultrasensitive methods have been evaluated with real patient samples, which is key to establishing clinical sensitivity and selectivity.Keywords
This publication has 126 references indexed in Scilit:
- Microarray-Based Multiplexed Scanometric Immunoassay for Protein Cancer Markers Using Gold Nanoparticle ProbesAnalytical Chemistry, 2009
- Designing nanomaterial-enhanced electrochemical immunosensors for cancer biomarker proteinsBioelectrochemistry, 2009
- Single-Wall Carbon Nanotube Forest Arrays for Immunoelectrochemical Measurement of Four Protein Biomarkers for Prostate CancerAnalytical Chemistry, 2009
- Bio/Abiotic Interface Constructed from Nanoscale DNA Dendrimer and Conducting Polymer for Ultrasensitive Biomolecular DiagnosisSmall, 2009
- Microsphere-Based Rolling Circle Amplification Microarray for the Detection of DNA and Proteins in a Single AssayAnalytical Chemistry, 2009
- Electrochemical immunosensors for interleukin-6. Comparison of carbon nanotube forest and gold nanoparticle platformsElectrochemistry Communications, 2009
- Carbon Nanotubes for Electronic and Electrochemical Detection of BiomoleculesAdvanced Materials, 2007
- Label‐Free Impedance Biosensors: Opportunities and ChallengesElectroanalysis, 2007
- Detection of Protein Biomarkers Using RNA Aptamer Microarrays and Enzymatically Amplified Surface Plasmon Resonance ImagingAnalytical Chemistry, 2007
- The Hallmarks of CancerCell, 2000