Spontaneous Mutations in Diploid Saccharomyces cerevisiae
- 1 December 2004
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 168 (4), 1817-1825
- https://doi.org/10.1534/genetics.104.033761
Abstract
We performed a 1012-generation mutation-accumulation (MA) experiment in the yeast, Saccharomyces cerevisiae. The MA lines exhibited a significant reduction in mean fitness and a significant increase in variance in fitness. We found that 5.75% of the fitness-altering mutations accumulated were beneficial. This finding contradicts the widely held belief that nearly all fitness-altering mutations are deleterious. The mutation rate was estimated as 6.3 × 10−5 mutations per haploid genome per generation and the average heterozygous fitness effect of a mutation as 0.061. These estimates are compatible with previous estimates in yeast.Keywords
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